蔡润佳,张静,黄建盛,陈刚,张健东,潘传豪,王忠良,谢瑞涛,汤保贵

• 1:广东海洋大学水产学院
• 2:南方海洋科学与工程广东省实验室(湛江)
• 3:广东恒兴饲料实业股份有限公司
• 4:农业农村部华南水产与畜禽饲料重点实验室

摘要(Abstract)：

【目的】探讨低温胁迫对军曹鱼(Rachycentroncanadum)幼鱼脂代谢相关生理生化的影响。【方法】将军曹鱼幼鱼分别在自然水温(30.5±1.0)℃(对照)、(20.0±0.5)℃条件下养殖7 d,并在1、4、7 d时采集各组血清、肝脏、肌肉和腹腔脂肪(intraperitoneal fat, IPF),测定血清甘油三酯(triglycerides, TG)、总胆固醇(total cholesterol, T-CHO)、高密度脂蛋白(high-density lipoprotein, HDL)、低密度脂蛋白(low-density lipoprotein, LDL)、丙二醛(malondialdehyde, MDA)和总抗氧化能力(total antioxidant capacity, T-AOC)等生化指标,以及肌肉、肝脏和IPF的脂肪酸组成。以油红O染色切片观察肝脏组织。【结果】低温胁迫过程中,军曹鱼血清TG含量随胁迫时间的延长呈上升趋势;T-CHO呈先降后升再降的趋势;HDL在前4 d与对照组无显著差异,之后显著低于对照组;LDL 1 d时与对照组无显著差异,之后呈显著上升趋势;血清T-AOC在7 d内均呈下降趋势,MDA在显著上升后又呈下降趋势;肝脏组织油红O切片结果显示,有脂滴分布不均的现象;低温胁迫对军曹鱼肌肉脂肪酸组成的影响不显著,但可显著提高肝脏和IPF的多不饱和脂肪酸(Polyunsaturated fatty acid, PUFA)比例。【结论】低温胁迫导致军曹鱼幼鱼机体抗氧化能力降低并积累大量MDA,军曹鱼从提高血清TG含量、增加血清胆固醇含量(增加LDL、减少HDL)、调整肝脏和IPF中脂肪酸组成方面响应低温胁迫。

关键词(KeyWords)： 军曹鱼;低温胁迫;脂代谢;生理生化

Abstract:

Keywords:

基金项目(Foundation): 南方海洋科学与工程广东省实验室(湛江)项目(ZJW-2019-06);; 湛江市海洋经济创新发展示范市项目(XM-202008-03);; 国家海水鱼产业技术体系项目(CARS-47-G08)

作者(Author): 蔡润佳,张静,黄建盛,陈刚,张健东,潘传豪,王忠良,谢瑞涛,汤保贵

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