冯子齐,阙凡迪,余方苗

• 1:浙江海洋大学食品与药学学院/浙江省海洋生物医用制品工程技术研究中心

摘要(Abstract)：

【目的】探究厚壳贻贝活性肽（Mytilus coruscus peptides,MCP）对酒精诱导小鼠肝损伤的保护及肠道菌群的调节作用。【方法】将45只C57BL/6小鼠平均分成5组：正常组、模型组（无水乙醇EtOH）、阳性组（EtOH+50 mg/kg水飞蓟素）、MCP低剂量组（EtOH+200 mg/kg MCP）和高剂量组（EtOH+400 mg/kg MCP）。LieberDeCarli液体饲料喂养C57BL/6小鼠3周建立酒精性肝损伤模型，检测小鼠血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性及肝脏中抗氧化酶活性和抗炎因子水平，通过H&E染色和油红O染色观察肝脏组织形态变化和脂肪堆积情况，采用16s rRNA测序检测小鼠肠道菌群多样性变化。【结果】MCP显著提高肝脏中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性，显著降低促炎因子肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)含量，同时降低了肝脏中甘油三酯（TG）和丙二醛（MDA）的含量（P <0.05）；减少肝脏脂肪空泡，增加结肠杯状细胞，恢复了肝细胞和结肠细胞正常结构。改善了由于酒精引起的肠道中操作分类单元（OTU）总数增加的情况，并且降低了革兰阴性菌的比例。【结论】MCP对小鼠酒精性肝损伤具有明显的保护作用，同时能调节小鼠肠道菌群的组成。

关键词(KeyWords)： 厚壳贻贝活性肽;酒精肝损伤;抗氧化;肠道菌群

Abstract:

Keywords:

基金项目(Foundation): 浙江省属高校基本科研业务费（2021JZ011）;; 浙江省基础研究公益计划项目（LGN20D060002）

作者(Author): 冯子齐,阙凡迪,余方苗

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