洪廷,任佳佳,张宁,李长玲,黄翔鹄

• 1:广东海洋大学水产学院
• 2:广东海洋大学深圳研究院
• 3:广东省藻类养殖及应用工程技术研究中心

摘要(Abstract)：

【目的】探究基于考马斯亮蓝染色法的波吉卵囊藻(Oocystis borgei)蛋白质提取方法,以快速测定波吉卵囊藻的蛋白质含量。【方法】以凯氏定氮法为基准,以考马斯亮蓝法测定经反复冻融法、超声波破碎法和碱提法提取的波吉卵囊藻(鲜品)蛋白含量,比较各提取方法的提取效果;通过单因素和响应面实验,考察碱提法中提取温度、NaOH浓度和提取时间对波吉卵囊藻蛋白提取率的影响。【结果】碱提法显著优于其他提取方法(P <0.01);碱提法中,各因素对波吉卵囊藻蛋白提取率的影响显著性由大到小依次为提取温度、NaOH浓度、提取时间;最佳提取条件:提取温度74.5℃、NaOH浓度0.35mol/L、提取时间69min,此时蛋白得率为1.726%,与模型预测值1.731%接近,以凯氏定氮法测定的藻体蛋白质含量为基准,蛋白提取率高达99.20%。【结论】碱提法是波吉卵囊藻(鲜品)蛋白的最佳提取方法,采用响应面法建立的模型可较好地预测提取温度、NaOH浓度和提取时间对波吉卵囊藻蛋白提取率的影响。

关键词(KeyWords)： 波吉卵囊藻;蛋白提取;快速提取;考马斯亮蓝法;响应面法

Abstract:

Keywords:

基金项目(Foundation): 深圳市大鹏新区产业发展专项资金“活性微藻制品产业化关键技术的研究与示范”

作者(Author): 洪廷,任佳佳,张宁,李长玲,黄翔鹄

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