叶星,王海英,简清,白俊杰,劳海华

• 1:中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室!广东广州510380
• 2:中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室
• 3:中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室
• 4:中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室

摘要(Abstract)：

<正>1966年Lin首次报道了将外源基因注入小鼠卵中的显微注射方法[1]。1982年诞生了首例转基因动物——转基因小鼠[2]。1984和1985年转基因虹鳟[3]和鲫鱼[4]研究获得成功。经过二十多年来的努力,转基因水生动物尤其是转基因鱼的研究有了长

关键词(KeyWords)： 转基因;水生动物;技术;应用;存在问题

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(2003C20310);; 广东省科技重点引导项目(2005B2031018)

作者(Author): 叶星,王海英,简清,白俊杰,劳海华

参考文献(References)：

• [1]Lin T P.Microinjection of mouse eggs[J].Science,1966,151:333-337.
• [2]Palmiter R D,Brinster R L,Hammer R E.Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes[J].Nature,1982,300:611-615.
• [3]Maclean N,Talwar S.Injection of cloned genes with rainbow trout eggs[J].J Embryol and Exp Morphol,1984,82:187-190.
• [4]Zhu Z Y,Li G,He L,et al.Novel gene transfer into the fertilized eggs of the goldfish Carassius acuratus[J].J Appl Ichthyol,1985,1:31-34.
• [5]楼允东.中国转基因鱼研究的进展[J].上海水产大学学报,1997,6(1):42-49.
• [6]Zbikowska H M.Fish can be first-advances in fish transgenesis for commercial application[J].Transgenic Res,2003,12(4):379-389.
• [7]朱作言,曾志强.转基因鱼离市场还有多远[J].生物技术通讯,2000(1):1-6.
• [8]Beardmore J A,Porter J S.Genetically modified organisms and aquaculture[J].FAO fisheries circular,2003,989:1-37.
• [9]朱作言,许克圣,谢岳峰,等.转基因鱼模型的建立[J].中国科学B,1989(2):147-155.
• [10]Maclean N,Rahman M A,Shom F,et al.Transgenic tilapia and the tilapia genome[J].Gene,2002,295:265-277.
• [11]Fu C,Hu W,Wang Y,et al.Developments in transgenic fish in the People's Republic of China[J].Rev Sci Tech OIE,2005,24(1):299-307.
• [12]Rajesh R,Majumdar K C.Transgene integration-an analysis in autotransgenic Labeo rohita Hamilton(Pisces:Cyprinidae)[J].Fish Physiol Biochem,2005,31:281-287.
• [13]Rahman M A,Mak R,Ayad H,et al.Expression of a novel piscine growth hormone gene results in growth enhancement in transgenic tilapia(Orechromis niloticus)[J].Transgenic Res,1998,7:357-369.
• [14]Devlin R H,Yesakl T Y,Biagl C A,et al.Extraordinary salmon growth[J].Nature,1994,371,6494:209-210.
• [15]Devlin R H,Yesaki T Y,Donaldson E M.Production of germline transgenic Pacific salmonids with dramatically increased growth performance[J].Can.J Fish Aquat Sci,1995,52(7):1376-1384.
• [16]Martinez R,Estrada M P,Berlanga J,et al.Growth enhancement in transgenic tilapia by ectopic expression of tilapia growth hormone[J].Mol Mar Biol Biotechnol,1996,5(1):62-70.
• [17]孙效文,梁利群,沈俊宝.全鱼基因工程鱼的构建[J].高科技通讯,1993,3(9):23-26.
• [18]Beardmore J A.Transgenics:autotransgenics and allotransgenics[J].Transgenic Res,1997,6:107-108.
• [19]Venugopal T,Anathy V,Kirankumar S,et al.Growth enhancement and food conversion efficiency of transgenic fish Labeo rohita[J].J Exp Zoolog A Comp Exp Biol,2004,301(6):477-490.
• [20]Morales R,Herrera M T,Arenal A.Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafis(Danio rerio)[J].Electronic J of Biotechnol,2001,4(2):52-58.
• [21]Nam Y K,Noh J K,Cho Y S,et al.Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis[J].Transgenic Res,2001,10(4):353-62.
• [22]Devlin R H,Biagl C A,Yesaki T Y.Growth,viability and genetic characteristics of GH transgenic coho salmon strains[J].Aquaculture,2004,236:607-632.
• [23]Fletcher G L,Hew C L,Davies P L.Antifreeze proteins of teleost fishes[J].Annu Rev Physiol,2001,63:359-390.
• [24]Hew C,Poon R,Xiong F,et al.Liver-specific and seasonal expres sion of transgenic Atlantic salmon harboring the winter flounder antifreeze protein gene[J].Transgenic Res,1999,8(6):405-414.
• [25]Wang R,Zhang P,Gong Z,et al.Expression of the antifreeze protein gene in transgenic goldfish(Carassius auratus)and its implication in cold adaptation[J].Mol Mar Biol Biotechnol,1995,4(1):20-26.
• [26]Wu S,Hwang P P,Hew C L.et al.Effects of antifreeze protein on cold tolerance in juvenile tilapia(Oreochromis mossambicus Peters)and milkfish(Channos channos Forskaal)[J].Zoological science,1998,37:39-44.
• [27]Hew C L,Jun D S,Gong Z,et al.Biotechnology for aquaculture:Transgenic salmon with enhanced growth and freeze-resistance[J].Biotechnol Apl,1996,13:49.
• [28]Marshall C B,Fletcher G L,Davies P L.Hyperactive antifreeze protein in fish[J].Nature,2004,429:153.
• [29]Sherry Y G,Chrimstopher B M,Garth L F.Hyperactive antifreeze protein in flounder species:The sole freeze protectant in American plaice[J].FEBS,2005,272:4439-4449.
• [30]Demers N E,Bayne C J.The immediate effects of stress on hormones and plasma lysozyme in rainbow trout[J].Developmental and comparative immunology,1997,21:363-373.
• [31]Dunham R A,Warr G W,Nichols A,et al.Enhanced bacterial disease resistance of transgenic channel catfish Ictalurus punctatus.ossessing cecropin genes[J].Marine biotechnology,2002,4(3):338-344.
• [32]Sarmasik A,Jang I K,Chun C Z,et al.Transgenic live-bearing fish and crustaceans produced by transforming immature gonads with eplication-defective pantropic retroviral vectors[J].Marine biotechnology,2001,3(5):470-477.
• [33]Yazawa R,Hirono I,Aoki T.Transgenic zebrafish expressing chicken lysozyme show resistance against bacterial diseases[J].Transgenic Res,2006,15(3):385-91.
• [34]Andrew D,Pauline M C,Liam C W,et al.Zebrafish:bridging the gap between development and disease[J].Human molecular genetics,2000,9(16):2443-2449.
• [35]Long Q,Meng A M,Wang Han,et al.GATA-1expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene[J].Development,1997,124:4105-4111.
• [36]Colin G M,Lesley R,Shirle I S,et al.Faithful expression of a tagged Fugu WT1protein from a genomic transgene in zebrafish:efficient splicing of pufferfish genes in zebrafish but not mice[J].Nucleic acids research,2003,31(11):2795-2802.
• [37]Gong Z,Wan H,Tay T L,et al.Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle[J].Biochem Biophy Res Commun,2003,308(1):58-63.
• [38]Jian Q,Bai J J,Ye X,et al.Construction of red fluorescence protein transgenic zebrafish[J].Anim Biotech Bulletin,2004,9(1):317-320.
• [39]王海英,叶星,白俊杰,等.三种鱼!-actin启动子的序列比较及唐鱼!-actin启动子功能的初步检测[J].实验动物科学与管理,2006,23(增刊):57-62.
• [40]Hwang G,Muller F,Rahman M A,et al.Fish as Bioreactors:Transgene expression of human coagulation factor VII in fish embryos[J].Mar Biotechnol(NY),2004,6(5):485-92.
• [41]Lu J K,Chen T T,Allen S K,et al.Production of transgenic dwarf surfclams,Mulinia lateralis,with pantropic retroviral vectors[J].Proc Natl Acad Sci,1996,93:3482-3486.
• [42]Burns J C,Chen T T.Pantropic retroviral vectors for gene transfer in mollusk:USA,5,969,211[P].1999.United stated Patent and Trademark Office,Alexandria,VA.
• [43]Tseng F S,Tsai H J,Liao I C.Introducing foreign DNA into tiger shrimp(Penaeus monodon)by electroporation[J].Theriogenology,2000,54(9):1421-1432.
• [44]Lu Y,Sun P S.Viral resistance in shrimp that express an antisense Taura syndrome virus coat protein gene[J].Antiviral Res,2005,67(3):141-146.
• [45]张达,朱厚础,黄留玉.细菌人工染色体及其在基因组学研究中的应用[J].生物技术通讯,2003,14(5):406-409.
• [46]王军平,张友明,粟永萍.Red/ET同源重组介导细菌人工染色体的快速修饰[J].生物化学与生物物理进展,2005,32(5):468-473.
• [47]Jenssen R J,Meng A,Ramsay J,et al.Modification of bacterial artificial chromosomes through Chistimulated homologous recombination and its application in zebrafish transgenesis[J].Genetics,1998,95:5121-5126.
• [48]Yang X W,Model P,Heintz N.Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome[J].Nat Biotechnol,1997,15(9):859-865.
• [49]Qiaoming L,Anming M,Han W,et al.GATA-1expression pattern can be recapitulated in linving transgenic zebrafish using GFP reporter gene[J].Development,1997,124:4105-4111.
• [50]Hong Y H,Gui J F,Chen S L,et al.Embryonic stem cells in fish[J].动物学报,2003,49(3):281-293.
• [51]陈松林.鱼类胚胎干细胞研究进展[J].中国水产科学,2001,7(4):95-98.
• [52]Howard R D,DeWoody J A,Muir W M.Transgenic male mating advantage provides opportunity for Trojan gene effect in a fish[J].Proc Natl Acad Sci,2004,101(9):2934-2938.
• [53]Bessey C,Devlin R H,Liley N R,et al.Reproductive performance of growth-enhanced transgenic coho salmon[J].Trans Am Fish Soc,2004,133(5):1205-1220.
• [54]Vandersteen T W E,Abrahams M V.Competitive ability and mortality of growth-enhanced transgenic Coho Salmon fry and parr when foraging for food[J].Trans Am Fish Soc,2005,134:381-389.
• [55]Sundstrom L F,Lohmus M,Johnsson J I.Growth hormone transge-nic salmon pay for growth potential with increased predation mortality[J].Proc Biol Sci,2004,271(Suppl5):350-362.
• [56]Uzbekova S,Chyb J,Ferriere F,et al.Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon[J].J Mol Endocrnol,2000,25(3):337-350.
• [57]朱作言.从转基因鱼研制谈自主创新研究[J].科技进步与对策,2001,18(1):14-16.
• [58]Kapuscinski A R.Current scientific understanding of the environmental biosafety of transgenic fish and shellfish[J].Rev Sci Tech OIE,2005,24(1):309-322.
• [59]曾北危.转基因生物安全[M].北京:化学工业出版社,2004:55-60.
• [60]Zhang F,Wang Y,Hu W,et al.Physiological and pathologyica analysis of mice fed with'all-fish'gene gransferred Yellow River carp[J].High Technol Lett,2000,10:17-19.