王肇鼎,李宁,白婵,王炬光,柴毅,熊光权

• 1:长江大学动物科学技术学院/农学院
• 2:湖北省农业科学院农产品加工与核农技术研究所/农业农村部农产品冷链物流技术重点实验室
• 3:湖北省农业科技创新中心农产品加工研究分中心
• 4:洪湖市农业农村局

摘要(Abstract)：

【目的】探讨CO_2麻醉辅助大口黑鲈（Micropterus salmoides）保活模拟运输时的温度、CO_2浓度和运输时间的选择，评价大口黑鲈模拟运输过程中血清生化指标和肌肉营养成分的变化。【方法】从暂养水箱中随机取20尾大口黑鲈放置在装有15 L曝气后的实验用水的玻璃鱼缸中，采用单因素实验，以存活率为指标，确定最佳运输温度和CO_2浓度；在最佳水温和CO_2质量浓度下进行保活模拟运输，分别在保活模拟运输的0、12、24、36和48 h取水样和血样，测定水质、血清生化指标、肌肉营养成分等指标。【结果】使用CO_2麻醉大口黑鲈，在7℃下需要15.23 min，在14℃下需要10.33 min。麻醉后在7℃水温中复苏时间为10.20 min,14℃时为3.53 min。经过单因素实验，确定最佳运输温度为15℃，最佳CO_2麻醉浓度为4.8、6.8 g/L，此条件下相较于只通入O_2的保活模拟运输，大口黑鲈存活率更好，肌肉营养更佳，且在模拟运输36 h时存活率为100%。模拟运输36 h时，血清中皮质醇、葡萄糖含量，乳酸脱氢酶活性均显著高于运输前（P<0.05）；谷草转氨酶、谷丙转氨酶活性，尿素氮、肌酐含量均显著低于运输前（P<0.05）；肌肉中乳酸含量显著高于运输前（P<0.05），糖原和蛋白质含量均显著低于运输前（P<0.05）。对照组血清中谷草转氨酶、谷丙转氨酶活性，尿素氮、肌酐含量显著高于CO_2处理组（P<0.05），说明CO_2麻醉可以减轻运输中应激造成的肝肾损伤。【结论】在运输温度为15℃，6.8 g/L CO_2条件下麻醉大口黑鲈进行保活模拟运输，应控制运输时间在36 h内。

关键词(KeyWords)： 大口黑鲈;保活运输;CO_2麻醉;运输效果

Abstract:

Keywords:

基金项目(Foundation): 财政部和农业农村部国家现代农业产业技术体系专项（CARS-46）;; 湖北省科技重大项目（2023BBA001）;; 湖北省农业科技创新中心重大科技研发专项（2020-620-000-002-03）;; 湖北省重点研发计划项目（2023BBB122）

作者(Author): 王肇鼎,李宁,白婵,王炬光,柴毅,熊光权

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