广东雷州地区3种滨珊瑚系统发生关系的研究Phylogenetic Relationships of Poritidae in Leizhou Based on12S rRNA Sequences
刘皓,骆学贤,周操,刘丽
摘要(Abstract):
对雷州常见的3种滨珊瑚科珊瑚:澄黄滨珊瑚(Porites lutea)、灰黑滨珊瑚(Porites nigrescens)、普哥滨珊瑚(Porites pukoensis),共32个样本的12S rRNA基因序列进行了PCR扩增和测序,研究其系统发生关系。结果显示,所得的3种珊瑚的12S rRNA基因长度在728~908 bp之间,G+C含量在34.3%~39.2%之间,遗传距离在0.004~0.019之间。利用邻接(NJ)法、最大似然(ML)法和不加权对群分析(UPGMA)法构建的系统进化树显示:3种珊瑚被分成了2个支系,澄黄滨珊瑚和灰黑滨珊瑚聚合在一个分支上,而普格滨珊瑚在另一支,这一研究结果与传统形态学分类研究的结果存在一定差异。
关键词(KeyWords): 滨珊瑚;12S rRNA;系统进化
基金项目(Foundation): 国家海洋公益性行业科研专项(201105012);; 广东省自然科学基金项目(S2011010000269);; 广东省海洋渔业科技推广专项(A201101B02)
作者(Author): 刘皓,骆学贤,周操,刘丽
参考文献(References):
- [1]邹仁林.中国动物志—腔肠动物门·珊瑚虫纲·石珊瑚目·造礁石珊瑚[M].北京:科学出版社,2001:1,27.
- [2]邹仁林.造礁石珊瑚[J].生物学通报,1998,33(6):8.
- [3]Daly M,Brugler M R,Cartwright P,et al.The phylum Cnidaria:A review of phylogenetic patterns and diversity 300 years after Linnaeus[J].Zootaxa,2007,1668:127-182.
- [4]Fukami H,Budd A F,Paulay G,et al.Conventional taxonomy obscures deep divergence between Pacific and Atlantic corals[J].Nature,2004,427(6977):832-835.
- [5]Forsman Z H,Concepcion G T,Haverkort R D,et al.Genetic and morphological characterization of a coral Species of Concern,Montipora dilatata,in Kaneohe Bay,Oahu,Hawaii[R].Hawaii Institute of Marine Biology:Pacific Islands Region Speices of Concern Project Report,2008:1-17.
- [6]刘丽,陈育盛,李晓娜.基于线粒体Cyt b基因的10种石珊瑚的系统发育关系[J].广东海洋大学学报,2011,31(1):6-11.
- [7]Saccone C,Gissi C,Lanave C,et al.Evolution of the mitochondrial genetic system:an overview[J].Gene,2000,261(1):153-159.
- [8]Liu H,Beckenbach A T.Evolution of the mitochondrial cytochrome oxidaseⅡgene among 10 orders of insects[J].Molecular Phylogenetics Evolution,1992,1(1):41-52.
- [9]Huang D,Meier R,Todd PA,et al,More evidence for pervasive paraphyly in scleractinian corals:Systematic study of Southeast Asian Faviidae(Cnidaria;Scleractinia)based on molecular and morphological data[J].Molecular Phylogenetics and Evolution,2009,50(1):102-116.
- [10]卢圣栋.现代分子生物学实验技术[M].第2版.北京:中国协和医科大学出版社,1999:61-66.
- [11]Chen C A,Carden C,Wallace,et al.Analysis of the mitochondrial 12S rRNA gene supports a two-clade hypothesis of the evolutionary history of scleractinian corals[J].Molecular Phylogenetics and Evolution,2002,23(2):137–149.
- [12]Lu G Q,Etsuko N M.Vector NTI,a balanced all-in-one sequence analysis suite[J].Brief Bioinformatics,2004,5(4):378-388.
- [13]Koichiro T,Daniel P,Nicholas P,et al.MEGA5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
- [14]Saccone C.The evolution of mitochondrial DNA[J].Current Opinion in Genetics&Development,1994,4(6)875-881.
- [15]刘海情,刘楚吾,刘丽.基于12S rRNA和16S rRNA序列研究龟鳖类的系统进化特征[J].南方农业学报,2012,43(11):1758-1764.
- [16]Hebert P D N,Penton E H,Burns J M,et al.Ten species in one:DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator[J].Proc Natl Acad Sci USA,2004,101:14812-14817.
- [17]李晓娜,刘丽,刘楚吾.运用线粒体COⅠ基因分析8种石珊瑚系统发育关系[J].渔业科学进展,2012,33(4),129-134.
- [18]刘丽,李晓娜,陈育盛,等.基于线粒体基因的石珊瑚分子系统学研究[J].海洋与湖沼,2012,43(4):814-820.
- [19]刘丽,李文娟,刘楚吾.广东徐闻地区滨珊瑚遗传多样性和系统发生关系[J].海洋与湖沼,2013,44(2):371-376.