范秀萍,张家胜,郭侨玉,杜欢,秦小明,刘书成

• 1:广东海洋大学食品科技学院//广东省水产品加工与安全重点实验室//广东省海洋食品工程技术研究中心//水产品深加工广东普通高等学校重点实验室
• 2:南方海洋科学与工程广东省实验室

摘要(Abstract)：

【目的】研究二氧化碳(CO_2)对珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×Epinephelus laceolatus♂)的麻醉效果,并探讨无水保活技术对石斑鱼运输的影响。【方法】以麻醉和复苏时间、无水保活时间和存活率为指标,探讨温度、CO_2浓度、麻醉时间、保活温度对珍珠龙胆石斑鱼CO_2麻醉后无水保活效果的影响;并对CO_2麻醉无水保活过程中的鱼体血清生化、游离氨基酸和基本营养成分进行分析。【结果】珍珠龙胆石斑鱼(503.0±37.0)g在25℃海水中暂养24 h后,逐渐降温至18℃,在含CO_2质量浓度125 mg/L(pH 5.8)的海水中麻醉5min,转移至15℃、充氧保湿的无水条件下保活,11 h后的存活率达到83.3%。低温CO_2麻醉与无水保活过程中鱼体代谢发生变化:谷丙转氨酶(ALT)、谷草转氨酶(AST)与尿素氮(BUN)水平均显著上升(P <0.05),有水复苏12 h后能恢复到正常水平;出现典型的"高血糖"症状。珍珠龙胆石斑鱼经低温CO_2麻醉、无水保活胁迫后主要表现出能量代谢的应激响应,以无氧呼吸为主,脂肪和糖原为主要能量物质;鲜味与苦味氨基酸含量显著下降(P <0.05),甜味氨基酸质量分数增加43.52%。保活后鱼肉营养品质优于低温休眠有水保活模式。【结论】CO_2对珍珠龙胆石斑鱼有较好的麻醉作用,CO_2麻醉协同低温可用于珍珠龙胆石斑鱼无水保活运输。

关键词(KeyWords)： CO_2麻醉;珍珠龙胆石斑鱼;无水保活;血清生化;代谢;游离氨基酸

Abstract:

Keywords:

基金项目(Foundation): “十三五”国家重点研发计划重点专项项目（2019YFD0901601）;; 南方海洋科学与工程广东省实验室（湛江）资助项目（ZJW-2019-06）;; 广东海洋大学科研启动经费资助项目（060302042101）;广东海洋大学创新团队项目（CXTD2021006）;; 广东省大学生创新创业训练计划项目（S201910566059）

作者(Author): 范秀萍,张家胜,郭侨玉,杜欢,秦小明,刘书成

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