田恒群,何康,郑平安,王琼芬,余方苗

• 1:浙江海洋大学食品与药学学院浙江省海洋生物医用制品工程技术研究中心
• 2:浙江海力生生物科技有限公司
• 3:浙江省舟山市食品药品检验检测研究院

摘要(Abstract)：

【目的】研究厚壳贻贝多肽对免疫抑制小鼠免疫功能的免疫调节作用。【方法】ICR小鼠随机分成给药组、空白组和模型组，其中给药组设3组，其厚壳贻贝免疫活性肽（MCP）质量分数分别为400、200、100mg/kg（即高、中、低剂量MCP组）；给药组和模型组小鼠注射环磷酰胺造成免疫缺陷，分别监测各组小鼠的体质量，并测定小鼠的免疫器官指数、脾细胞的转化增殖、迟发型超敏反应、自然杀伤细胞活性、腹腔巨噬细胞的吞噬能力、血清溶血素水平、血清中免疫球蛋白和细胞因子的水平。【结果与结论】与模型组相比，喂养高剂量MCP的免疫抑制小鼠体质量恢复，为39.62 g；胸腺和脾脏指数指数提高，分别为134.75%和128.31%；脾淋巴细胞、自然杀伤细胞和腹腔巨噬细胞的活性显著提高（P <0.05），分别为126.84%、224.55%和147.67%。此外，高剂量的MCP还能改善免疫抑制小鼠血清溶血素和免疫球蛋白含量，其中Ig A、Ig G、Ig M的质量浓度为308.82、1 322.5和235.88 pg/mL。,与模型组相比，高剂量组MCP提高了TNF-α、IL-2和IFN-γ的水平，分别为140.29%、127.95%和255.11%，降低了IL-4和IL-10的水平，分别为71.40%和72.64%。这表明MCP能增强免疫缺陷小鼠的免疫功能。

关键词(KeyWords)： 厚壳贻贝;多肽;免疫调节;免疫器官指数

Abstract:

Keywords:

基金项目(Foundation): 浙江省属高校基本科研业务费（2021JZ011）

作者(Author): 田恒群,何康,郑平安,王琼芬,余方苗

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