太平洋鳕仔鱼期消化酶活性的变化Development of Digestive Enzymes in Gadus macrocephalus Larvae
宫雪,姜志强,吴洪,范瑞,李艳秋
摘要(Abstract):
用酶学分析法研究太平洋鳕仔鱼发育过程中胰蛋白酶、酸性蛋白酶、淀粉酶和脂肪酶活性的变化。结果表明:4种酶的比活力均在初孵时即可检出,但比活力变化趋势不同;胰蛋白酶比活力在仔鱼孵化初期较高,随后显著降低;酸性蛋白酶比活力在早期仔鱼阶段比较低,且较为稳定,从14日龄开始显著升高;淀粉酶比活力在3日龄即达到最大值,之后显著降低,并一直保持较低的比活力;脂肪酶的比活力一直较低,在5日龄达到最大值,之后比活力大幅度下降。仔鱼发育过程中,各种酶活性变化显著(p<0.05),太平洋鳕在仔鱼期已经可以消化蛋白质、脂肪和碳水化合物。
关键词(KeyWords): 太平洋鳕;仔鱼;酸性蛋白酶;胰蛋白酶;淀粉酶;脂肪酶
基金项目(Foundation): 国家高技术研究发展计划(863计划)(2012AA10A413)
作者(Author): 宫雪,姜志强,吴洪,范瑞,李艳秋
参考文献(References):
- [1]高天翔,张肖荣,王丹,等.几种鳕鱼的生物学初步研究[J].海洋湖沼通报,2003(1):35-42.
- [2]Darowski K.The feeding of fish larvae:present state of art andperspectives[J].Reprod Nutr Develop,1984,24(6):807-833.
- [3]Lauff M,Hofer R.Proteolytic enzymes in fish development andthe importance of dietary enzymes[J].Aquaculture,1984,37(4):335-346.
- [4]陈慕雁,张秀梅,连建华.大菱鲆仔稚鱼期消化酶及碱性磷酸酶活性的变化[J].中国海洋大学学报,2005,35(3):483-486.
- [5]吴仁协,洪万树,张其永,等.大弹涂鱼仔稚鱼和早期幼鱼的消化酶活性[J].水产学报,2006,30(6):733-738.
- [6]于海瑞.大黄鱼仔稚鱼消化生理、蛋白质和蛋氨酸需要量的研究[D].青岛:中国海洋大学,2006.
- [7]Alvarez-González C A,Moyano-López F J,Civera-Cerecedo R,etal.Development of digestive enzyme activity in larvae of spottedsand bass Paralabrax maculatofasciatus.1.Biochemical analysis[J].Fish Physiology Biochemistry,2008,34(4):373-384.
- [8]Lazo J P,Mendoza R,Holt G J,et al.Characterization of digestiveenzymes during larval development of red drum(Sciaenopsocellatus)[J].Aquaculture,2007,265(1-4):194-205.
- [9]Ribeiro L,Sarasquete C,Dinis M T.Histological andhistochemical development of the digestive system of Soleasenegalensis(Kaup,1858)larvae[J].Aquaculture,1999,171(3-4):293-308.
- [10]Gisbert E,Gimenez G,Fernandez I,et al.Development ofdigestive enzymes in common dentex Dentex dentex during earlyontogeny[J].Aquaculture,2009,287(3-4):381-387.
- [11]Oozeki Y,Bailey K M.Ontogenetic development of digestiveenzyme activities in larval walleye pollock,Theragrachalcogramma[J].Marine Biology,1995,122(2):177-186.
- [12]桂远明.水产动物机能学实验[M].北京:中国农业出版社,2004:119-124.
- [13]Murray H M,Gallant J W,Johnson S C,et al.Cloning andexpression analysis of three digestive enzymes from Atlantichalibut(Hippoglossus hippoglossus)during early development:Predicting gastrointestinal functionality[J].Aquaculture,2006,252(2-4):394-408.
- [14]Gisbert E,Piedrahita R H.Ontogenetic development of thedigestive system in California halibut(Paralichthys californicus)with notes on feeding practices[J].Aquaculture,2004,232(1-4):455-470.
- [15]Ma H,Cahu C,Zambonino J,et al.Activities of selecteddigestive enzymes during larval development of large yellowcroaker(Pseudosciaena crocea)[J].Aquaculture,2005,245(1-4):239-248.
- [16]Pedersen B H.Growth and mortality in young larval herring(Clupea harengus):Effects of repetitive changes in foodavailability[J].Mar Biol,1993,117(4):547-550.
- [17]Zambonino Infante J L,Cahu C L.Dietary modulation of somedigestive enzymes and metabolic processes in developing marinefish:Applications to diet formulation[J].Aquaculture,2007,268(1-4):98-105.
- [18]Comabella Y,Mendoza R,Aguilera C,et al.Digestive enzymeactivity during early larval development of the Cuban garAtractosteus tristoechus[J].Fish Physiology and Biochemistry,2006,32(2):147-157.
- [19]Gawlicka A,Teh S J,Hung S S O,et al.Histological andhistochemical changes in the digestive tract of white sturgeonlarvae during ontogeny[J].Fish Physiology Biochemistry,1995,14(5):357-371.
- [20]Lundstedt L M,Melo JFB,Moraes G.Digestive enzymes andmetabolic profile of Pseudoplatystoma corruscans(Teleostei:Siluriformes)in response to diet composition[J].ComparativeBiochemistry and Physiology,2004,137(3):331-339.
- [21]刘铭,胡先成,韩强,等.河川沙塘鳢胚胎、仔鱼发育过程中蛋白酶活性的变化[J].淡水渔业,2008,38(5):39-41.
- [22]Perez-Casanova J C,Murray H M,Gallant J W.Development ofthe digestive capacity in larvae of haddock(Melanogrammusaeglefinus)and Atlantic cod(Gadus morhua)[J].Aquaculture,2006,251(2-4):377-401.
- [23]Douglas S E,Mandla S,Gallant J W.Molecular analysis of theamylase gene and its expression during the development in thewinter flounder,Pleuronectes americanus[J].Aquaculture,2000,190(3-4):247-260.
- [24]Ma P,Sivaloganathan B,Reddy P K,et a1.Ontogeny ofalpha-amylase gene expression in Sea Bass Larvae(Latescalcarifer)[J].Marine Biotechnology,2001,3(5):463-469.
- [25]Cahu C L,Zambonino Infante JLZ.Early weaning of Sea bass(Dicentrarchus labrax)larvae with a compound diet:Effect ondigestive enzymes[J].Comparative Biochemisty and Physiology,1994,109(2):213-222.
- [26]Ribeiro L,Zambonino-Infante J L,Cahu C,et a1.Developmentof digestive enzymes in larvae of Solea senegalensis,Kaup 1858[J].Aquaculture,1999,179(1-4):465-473.
- [27]Meeren,Tvan der.Algae as first food for cod larvae,Gadusmorhua L.:Filter feeding or ingestion by accident?[J].Journal ofFish Biology,1991,39(2):225-237.