基于OSMAC策略的3株海洋真菌发酵条件筛选与优化Screening and Optimization of Fermentation Conditions on Three Marine Fungi Based on the OSMAC Strategy
刘亚月,梁健文,薛欣怡,梁金月,马小翔,张翼
摘要(Abstract)：
【目的】筛选可促进海洋真菌产生代谢产物更丰富的发酵条件,进一步挖掘菌株在抗阿尔茨海默症方面的潜力。【方法】基于OSMAC策略,分别选用马铃薯葡萄糖水(PDB)培养基、察氏培养基、大米培养基和葡萄糖/蛋白胨/酵母膏(GPY)培养基,并设置3种盐度(3、30和100),对Talaromyces sp. 001、Penicillium sp. 019和Aspergillus terreus ZN4-5-4等3株海洋真菌进行小规模发酵培养。通过观察真菌在培养基中的生长状态变化,并根据TLC指纹图谱、乙酰胆碱酯酶(AChE)抑制、1, 1-二苯基-2-三硝基苯肼(DPPH)自由基清除和卤虫致死活性筛选模型等技术手段筛选出代谢产物丰富、毒性小且AChE抑制活性与抗氧化活性产物丰富的发酵培养条件。【结果】菌株001的最优发酵条件是盐度3的GPY培养基,菌株019的最优发酵条件是盐度3的PDB培养基,菌株ZN4-5-4的最优发酵条件是盐度3的PDB培养基。【结论】OSMAC策略指导下,改变培养基条件对菌株产生次生代谢产物有显著影响。
关键词(KeyWords)： OSMAC;海洋真菌;次生代谢产物;抗乙酰胆碱酯酶;抗氧化
基金项目(Foundation): 国家自然科学基金(21807015);; 广东海洋大学博士启动项目(R18008);; 广东省扬帆计划引进紧缺拔尖人才项目(201433009);; 广东省自然科学基金(2018A030307046);; 深圳市科创委基础研究自由探索项目(JCYJ20170306165013264);; 广东省应用科技研发重大专项(2016B020235001);; 广东省促进经济发展专项资金(海洋经济发展用途)项目(粤自然资源合[2019] 015号);; 深圳市大鹏新区产业发展资金(KY20180203及PT201901-05);; 湛江市海洋渔业局项目(A18018)
作者(Author): 刘亚月,梁健文,薛欣怡,梁金月,马小翔,张翼
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