聂一帆,吴静,马艳平,郝乐,冯国清,刘振兴,曹俊明

• 1:仲恺农业工程学院动物科技学院
• 2:广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室农业农村部兽用药物与诊断技术广东科学观测实验站
• 3:华南农业大学兽医学院
• 4:广东海洋大学

摘要(Abstract)：

【目的】克隆大口黑鲈(Micropterus salmoides)肿瘤坏死因子-α诱导蛋白2 (Tumor necrosis factor-alpha inducedprotein2,TNFAIP2)基因，命名为Ms TNFAIP2，探究Ms TNFAIP2在大口黑鲈免疫应答中的作用。【方法】根据转录组中Ms TNFAIP2编码序列（CDS）设计引物并克隆该基因；利用DNAMAN等软件和SMART等预测并分析Ms TNFAIP2氨基酸序列理化性质和结构；用实时荧光定量PCR分析在鰤鱼诺卡氏菌(Nocardia seriolae)和脂多糖刺激前后Ms TNFAIP2及TNF-α、IL-1β、IL-10和IFN-γ的m RNA表达变化。【结果】成功克隆Ms TNFAIP2 CDS序列，长度为1 932 bp，共编码644个氨基酸，有一个Sec6超家族结构域；与鳜(Siniperca chuatsi)TNFAIP2氨基酸序列同源性最高，为74.84%；鰤鱼诺卡氏菌和脂多糖刺激后，Ms TNFAIP2及TNF-α、IL-1β、IL-10和IFN-γ m RNA表达水平在头肾、脾脏和淋巴细胞中显著上调(P <0.05)。【结论】Ms TNFAIP2参与了大口黑鲈免疫应答。

关键词(KeyWords)： 大口黑鲈;肿瘤坏死因子-α诱导蛋白2;基因克隆;基因表达;脂多糖;鰤鱼诺卡氏菌

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金(2022A1515012556,2021A1515010498);; 佛山市财政专项资金-2020年度共建广东农业科技示范市项目;; 广东省农业科学院动物卫生研究所创新基金（CX202113);广东省农业科学院水产协同创新中心项目（XT202232）

作者(Author): 聂一帆,吴静,马艳平,郝乐,冯国清,刘振兴,曹俊明

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