李晋南,王常安,王连生,赵志刚,罗亮,徐奇友

• 1:中国水产科学研究院黑龙江水产研究所

摘要(Abstract)：

【目的】探讨低磷饲料中添加谷氨酸对松浦镜鲤肠道消化酶活性及肠道形态的影响。【方法】将初始体质量(5.07±0.02)g的松浦镜鲤450尾随机分为5组,其中4个实验组在低磷(NaH_2PO_3质量分数1.5%)饲料中分别添加质量分数0%、0.5%、1.0%和2.0%的谷氨酸(Glu),分别为G0、G0.5、G1.0、G2.0组,同时以正常磷饲料(2.0%NaH2PO3)为对照(C组),实验周期为8周。【结果】G1组前肠和后肠的蛋白酶活性显著高于G0组,与C组差异不显著;G1前肠脂肪酶活性显著高于G0、G0.5和G2组,C和G1组中肠脂肪酶活性显著高于G0组;C和G1组中肠淀粉酶活性显著高于其他各组(P <0.05),各组间前肠和后肠淀粉酶活性差异不显著(P <0.05)。各添加组的松浦镜鲤幼鱼前肠和后肠的皱襞高度显著高于G0组,且前肠的皱襞高度与C组相比差异不显著;C组后肠的皱襞高度显著高于其他各组(P <0.05)。G2组前肠的绒毛宽度与C组相比差异不显著(P>0.05),其他各组的松浦镜鲤幼鱼前肠的绒毛宽度显著低于C组(P<0.05);中肠和后肠的绒毛宽度各组间没有显著差异(P> 0.05)。各添加组的松浦镜鲤幼鱼前肠的基层厚度与C组比没有显著差异(P> 0.05)。中肠和后肠的基层厚度各组间没有显著差异(P> 0.05)。肠体指数和肠长指数各组间没有显著差异(P> 0.05)。添加Glu组的前肠绒毛高度及密度均显著增加,且排列整齐。【结论】低磷饲料中添加谷氨酸可以增加松浦镜鲤幼鱼肠道消化酶活性并改善肠道形态结构。

关键词(KeyWords)： 松浦镜鲤幼鱼;谷氨酸;磷;消化酶;肠道形态

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项(HSY201605);; 国家自然科学基金项目(31702353);; 黑龙江省优秀青年基金项目(YQ2019C035);; 现代农业产业技术体系建设专项(CARS-45);; 黑龙江省应用技术研究与开发计划重大项目(GA18B202)

作者(Author): 李晋南,王常安,王连生,赵志刚,罗亮,徐奇友

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