郝雅茹,高加龙,曹文红,秦小明,苏伟明

• 1:广东海洋大学食品科技学院/国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/水产品深加工广东普通高等学校重点实验室

摘要(Abstract)：

【目的】应用蛋白组学技术解析镉（Cd）、硒（Se）胁迫对华贵栉孔扇贝（Chlamys nobilis）组织中蛋白表达的影响，为扇贝金属蓄积和耐受机制研究提供数据。【方法】将华贵栉孔扇贝置于分别含有Cd、Se、Cd+Se的海水中，采用非标记定量Label-free蛋白组学技术分析对照组和处理组扇贝消化腺的差异表达蛋白（differentially expressed proteins,DEPs），并对其进行GO富集、KEGG通路富集和蛋白质相互作用网络分析。【结果】CT组的闭壳肌和外套膜中的Cd质量分数显著高于鳃、性腺和消化腺（P <0.05）。除闭壳肌外，在镉、硒、Cd+Se胁迫下扇贝其他组织中的Cd或Se质量分数均显著增加（P <0.05）。Se+Cd胁迫下扇贝闭壳肌、外套膜和消化腺中的Se质量分数显著降低（P <0.05），说明扇贝中Se和Cd相互拮抗。共鉴定出887个DEPs；与对照组相比，Se组45个上调蛋白、9个下调蛋白，Cd组15个上调蛋白、10个下调蛋白，CdSe组13个上调蛋白、3个下调蛋白。生物信息学分析发现，Cd、Se胁迫的DEPs主要涉及参与细胞过程、代谢过程和生物调节，这些过程与KEGG通路分析所强调的信号通路有关。【结论】Cd、Se胁迫影响华贵栉孔扇贝消化腺多种蛋白的表达，导致相关代谢紊乱。

关键词(KeyWords)： 华贵栉孔扇贝;镉;硒;胁迫;非标记定量蛋白组学

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金（2017A030310626）;; 广东海洋大学科研启动经费资助项目;; 贝类产业技术体系项目（CARS-49）

作者(Author): 郝雅茹,高加龙,曹文红,秦小明,苏伟明

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