李亚,陈燕玲,鲍敏丽,许美容,邓晓玲

• 1:广东海洋大学农学院
• 2:华南农业大学农学院/广东省微生物信号与作物病害防控重点试验室

摘要(Abstract)：

【目的】克隆长春花(Catharanthus roseus)铜锌超氧化物歧化酶(Cu/Zn superoxide dismutase, Cu/Zn-SOD)基因,研究黄龙病菌("CandidatusLiberibacterasiaticus")侵染后长春花Cu/Zn-SOD基因(Cr Cu/Zn-SOD)的表达。【方法】根据不同作物Cu/Zn-SOD基因序列同源性筛选出CrCu/Zn-SOD基因引物,扩增得Cr Cu/Zn-SOD基因片段,用SMART-RACE方法扩增出Cr Cu/Zn-SOD基因的3′和5′末端,用q RT-PCR分析嫁接后不同时间长春花叶、主茎和根中Cr Cu/Zn-SOD基因的m RNA转录水平。【结果】拼接后获得Cr Cu/Zn-SOD基因全长796 bp(Genbank登录号KP864639),该基因与其他物种的Cu/Zn-SOD基因同源性达86%,编码1个亲水性稳定蛋白,无跨膜结构域及N末端无信号肽。染病长春花叶、茎和根中,CrCu/Zn-SOD的表达在侵染25 d前均上调,30～45 d时表达量持续下降,45 d时叶、茎和根中表达量分别下调1.8倍、2.97倍和2.9倍。【结论】长春花Cu/Zn-SOD序列与其他物种同源性较高,Cr Cu/Zn-SOD表达受黄龙病菌侵染诱导,表达量变化与长春花抗黄龙病菌侵染能力密切相关。

关键词(KeyWords)： 长春花;铜锌超氧化物岐化酶;基因克隆;黄龙病菌;基因表达

Abstract:

Keywords:

基金项目(Foundation): 广东海洋大学博士启动(R17060);广东海洋大学校选课题(C17385)

作者(Author): 李亚,陈燕玲,鲍敏丽,许美容,邓晓玲

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