林可鑫,李锦锋,陈明珠,黄日辉,徐琼瑜,郭晖,冯雪敏,彭东俊,李广丽,石红娟,朱春华

• 1:广东海洋大学水产学院/广东省名特优鱼类生殖调控与繁育工程技术研究中心
• 2:南方海洋科学与工程广东省实验室(湛江)

摘要(Abstract)：

【目的】探究视黄酸受体（RAR）介导视黄酸（RA）信号的作用和分子机制，以及对尼罗罗非鱼（Oreochromis niloticus）生殖调控的影响，为理解RAR介导RA信号在鱼类生殖调控中的分子机制提供理论基础。【方法】利用50 nmol/L RAR抑制剂AGN193109分别对尼罗罗非鱼精巢和卵巢组织离体孵育48 h，采用转录组分析探究RAR抑制剂AGN193109对尼罗罗非鱼性腺的影响。【结果】在离体孵育精巢中筛选到339个差异表达基因，其中192个上调，147个下调；在离体孵育卵巢中筛选到148个差异表达基因，其中103个上调，45个下调。此外，在离体孵育精巢和卵巢中，共筛选出13个共表达差异基因。GO和KEGG富集分析显示，差异表达基因显著富集在生殖和生殖过程、代谢过程、MAPK信号通路、TGF-β信号通路、视黄醇代谢和类固醇激素合成等通路。qRT-PCR结果显示，在精巢中，RAR抑制剂AGN193109处理显著下调了视黄醇代谢相关基因（cyp26b1、cyp1a、dhrs3b）及精子发生相关基因（tdgf1、tncb、sox8）的表达。在卵巢中，AGN193109处理显著上调了卵泡发育相关基因（IL-1β、tnfa、mapkapk3、fstl3）及排卵相关基因（ptgs2b）的表达。α=0.05。【结论】RAR介导的RA信号可能通过下调视黄醇代谢相关基因（cyp26b1、cyp1a、dhrs3b）的表达，同时影响尼罗罗非鱼精子发生（tdgf1、tncb、sox8）和卵泡发育（IL-1β、tnfa、mapkapk3、fstl3）相关基因的表达，进而影响尼罗罗非鱼的生殖活动。

关键词(KeyWords)： 尼罗罗非鱼;视黄酸;视黄酸受体;视黄酸受体抑制剂;性腺发育;转录组分析

Abstract:

Keywords:

基金项目(Foundation): 广东省基础与应用基础研究基金（2023A1515012880）

作者(Author): 林可鑫,李锦锋,陈明珠,黄日辉,徐琼瑜,郭晖,冯雪敏,彭东俊,李广丽,石红娟,朱春华

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