10种CpG-ODNs对罗非鱼抗海豚链球菌感染的作用Effects of Injecting 10 Kinds of CpG ODNs on the Resistance of Tilapia (Oreochromis niloticus) against Streptococcus iniae
黄婷,张彬,陈福艳,陈明,雷爱莹,王瑞,余晓丽,罗永巨,梁万文,李昭信
摘要(Abstract)：
采用人工合成的10种未甲基化CpG-ODNs序列分别注射健康的吉富罗非鱼Oreochromis niloticus,48 h后注射海豚链球菌(Streptococcus iniae)进行人工感染,分别于感染24 h和48 h后检测鱼体血清溶菌酶(Lysozyme,LSZ)、超氧化物歧化酶(Superoxide dismutase,SOD)和碱性磷酸酶(Alkaline phosphatase,AKP)的活性,并记录死亡率。结果显示:注射ODN-205的罗非鱼LSZ、AKP和SOD活性变化均不显著(P>0.05),推测注射ODN-205后罗非鱼能较好的维持机体的平衡,增强机体的抗菌能力;注射ODN-1681、1670、2133和2006的罗非鱼LSZ活性逐渐降低,而注射ODN-1826、2143、1668、2102、1669的罗非鱼LSZ活性呈现先升高后降低的趋势。AKP活性的变化趋势同LSZ活性变化大体相似,而SOD活性前后变化不显著(p值>0.05)。注射CpG-ODNs的实验组抑制海豚链球菌感染的作用差异显著(p值<0.05),其中以注射ODN-205组的死亡率最低(18%),其次为注射ODN-1670、1681、2006和2133组,死亡率均低于30%,而ODN-1669组和生理盐水组的死亡率均超过90%。实验表明,注射一定量的ODN-205、1670、1681、2006和2133能明显提高吉富罗非鱼抗海豚链球菌感染能力。
关键词(KeyWords)： 吉富罗非鱼;CpGODNs;海豚链球菌;抗感染
基金项目(Foundation): 现代农业产业技术体系专项资金资助项目国家罗非鱼产业技术体系(CARS-49);; 广西科技计划项目(桂科攻10100014-1,桂科合10100019-16);; 广西科学基金项目(桂科青0991021)
作者(Author): 黄婷,张彬,陈福艳,陈明,雷爱莹,王瑞,余晓丽,罗永巨,梁万文,李昭信
参考文献(References)：
- [1]郭恩彦,郭忠宝,罗永巨.吉富罗非鱼最适生长水温研究[J].广东海洋大学学报,2011,31(1):88-93.
- [2]杨弘,单航宇,李大宇,等.罗非鱼产业发展中的热点问题探讨[J].中国水产,2011,2:16-18.
- [3]Ballas Z K,Rasmussen W L,Krieg A M.Induction of NK activity inmurine and human cells by CpG motifs in oligodeoxynucleotides andbacterial DNA[J].J.Immunol,1996,157:1840-1845.
- [4]Liang H,Nishioka Y,Reich C F,Pisetsky D S,et al.Activation ofhuman B cells by phosphorothioate oligodeoxynucleotides[J].J.ClinInvest,1996,98:1119-1129.
- [5]Meng Z,Shao J,Xiang L.CpG oligodeoxynucleotides activate grasscarp(Ctenopharyngodon idellus)macrophages[J].Dev.CompImmunol,2003,27:313-321.
- [6]Carrington A C,Collet B,Holland J W,et al.CpGoligodeoxynucleotides stimulate immune cell proliferation but notspecific antibody production in rainbow trout(Oncorhynchus mykiss)[J].Vet.Immunol Immunopathol,2004,101:211-222.
- [7]Carrington A C,Secombes J C.CpG oligodeoxynucleotidesup-regulate antibacterial systems and induce protection againstbacterial challenge in rainbow trout(Oncorhynchus mykiss)[J].FishShellfish Immunol,2007,23:781-792.
- [8]Rhodes L D,Rathbone C K,Corbett S C,et al.Efficacy of cellularvaccines and genetic adjuvants against bacterial kidney disease inchinook salmon(Oncorhynchus tshawytscha)[J].Fish ShellfishImmunol,2004,16:461-474.
- [9]Liu Chun-sheng,Sun Yun,Hua Yong-hua,et al.Identification andanalysis of a CpG motif that protects turbot(Scophthalmus maximus)against bacterial challenge and enhances vaccine-induced specificimmunity.Vaccine,2010,28:4153-4161.
- [10]Carrington A C,Secombes C J.A review of CpGs and their relevanceto aquaculture[J].Veterinary immunology and immunopathology,2006,112:87-101.
- [11]孙峰,张煜,李立德,等.感染嗜水气单胞菌对鲫鱼非特异性免疫功能的影响[J].中国海洋大学学报,2005,35(5):815-818.
- [12]Allison C,Christopher J.CpG oligodexynucleotides up-regulateantibacterial systems and induce protection against bacterialchallenge in rainbow trout(Oncorhynchus mykiss)[J].Fish andshellfish immunology,2007,23:781-792.
- [13]Tassakka ACMAR,Sakai M.CpG Oligodeoxynucleotides enhancethe non-specific immune responses on carp,Cyprinus carpio[J].Aquaculture,2002,209:1-10.
- [14]王文博,李爱华.环境胁迫对鱼类免疫系统影响的研究概况[J].水产学报,2002,26(4):368-374.
- [15]Winston GW,Di Giulio R T.Prooxidant and antioxidantmechanismin aquatic organism[J].Aquat Toxico,1991,l24:143-152.
- [16]李红霞,李义,刘永贵,等.CpG寡脱氧核苷酸对中华绒螯蟹3种血清酶活性的影响[J].饲料工业,2007,39(1):252-257.
- [17]马骞,洪婧妮,蒋秋芬,等.哈维氏弧菌对条纹斑竹鲨4种酶活性的影响[J].台湾海峡,2010,29(2):196-204.
- [18]郭伟荣,刘利平,张宗峰,等.感染鳗弧菌对花鲈非特异性免疫功能的影响[J].上海海洋大学学报,2011,20(1):89-95.