董忠典,宦章,黎学友,陈月碧,张宁,郭昱嵩,王中铎,梁燕秋

• 1:广东海洋大学水产学院/南海水产经济动物增养殖重点实验室
• 2:广东省水产动物病害防控与健康养殖重点实验室
• 3:广东海洋大学化学与环境学院

摘要(Abstract)：

【目的】研究低浓度炔诺酮（Norethisterone,NET）短期暴露对海洋青鳉（Oryzias melastigma）仔鱼的毒性效应。【方法】海洋青鳉仔鱼在8.96 ng/L的NET暴露10 d，分析仔鱼游泳行为，氧化-抗氧化相关酶活性，性激素、甲状腺激素水平，以及基因转录模式；NET处理后仔鱼在人工海水中饲养至5月龄，统计性别比例。【结果】NET短期暴露后，海洋青鳉仔鱼的游泳行为有显著的抑制效应，仔鱼在静止状态的时间减少，处于小运动和大运动状态的时间极显著增加（P <0.01）；丙二醛（MDA）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）的活性变化不显著（P> 0.05）；仔鱼雌二醇（E2）水平显著降低（P <0.05），睾酮（T）水平极显著提升（P <0.01），群体中雌雄比例无影响；132个基因的转录模式发生改变，主要涉及节律调节、物质代谢和免疫等生物学过程。【结论】海洋青鳉在低浓度NET环境短期暴露可引起仔鱼游泳行为、激素水平及基因转录模式的变化；海洋环境残留的NET对鱼类健康有潜在风险，进而破坏海洋渔业资源。

关键词(KeyWords)： 海洋青鳉;仔鱼;炔诺酮;毒性;行为;抗氧化酶;性激素;甲状腺激素;转录组

Abstract:

Keywords:

基金项目(Foundation): 广东海洋大学南海学者青年项目（QNXZ201903）;; 国家自然科学基金（41907346）

作者(Author): 董忠典,宦章,黎学友,陈月碧,张宁,郭昱嵩,王中铎,梁燕秋

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