张旭丽,简纪常,王蓓,蔡双虎,黄郁葱,鲁义善,吴灶和

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点试验室暨广东省高等学校水产经济动物病害控制重点实验室

摘要(Abstract)：

研究了海豚链球菌(Streptococcus iniae)ZJMX04的培养与生长特性,探讨培养基的pH值、气体环境、温度等因素对海豚链球菌生长的影响,并采用正交实验法对海豚链球菌培养基生长因子添加量进行优化。结果表明,海豚链球菌培养基的最适pH为7～8,最适培养温度为30℃,振荡培养对海豚链球菌的生长影响不明显。培养基优化研究表明,在BHI培养基中添加10mg/mL的酵母粉和2mg/mL的葡萄糖即可在对数期获得最大细菌量。

关键词(KeyWords)： 海豚链球菌(Streptococcus iniae);生长规律;培养条件优化

Abstract:

Keywords:

基金项目(Foundation): 广东省科学技术厅社会发展项目“主要海水养殖鱼类弧菌病流行病学研究”(2006A20301003)

作者(Author): 张旭丽,简纪常,王蓓,蔡双虎,黄郁葱,鲁义善,吴灶和

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