一种快速鉴定弓背青鳉遗传性别的方法A Method for Rapid Identification of Genetic Sex of Oryzias curvinotus
董忠典,龙水生,黄承勤,黄顺楷,张宁,应子钰,郭昱嵩,王中铎
摘要(Abstract)：
【目的】研究快速鉴定弓背青鳉遗传性别的方法。【方法】以中国南海沿岸弓背青鳉群体为对象提取基因组DNA,根据基因组数据设计性别特异引物Ocsex-F/Ocsex-R,通过PCR方法对弓背青鳉遗传性别进行鉴定。【结果】引物Ocsex-F/Ocsex-R在雄性DNA中扩增获得959 bp和656 bp的两个DNA条带,在雌鱼中仅可扩增出959 bp的单一条带,PCR产物经电泳可清晰分辨;对汕头、湛江、钦州、文昌等南海沿岸的地理群体扩增结果一致。【结论】Ocsex-F/Ocsex-R引物可对弓背青鳉进行遗传性别鉴定,在南海沿岸不同群体中具有稳定性和通用性,可广泛用于弓背青鳉遗传性别鉴定。
关键词(KeyWords)： 弓背青鳉;性别鉴定;分子标记
基金项目(Foundation): 国家自然科学基金项目(31201996);; 广东省人才项目(Yq2013089)
作者(Author): 董忠典,龙水生,黄承勤,黄顺楷,张宁,应子钰,郭昱嵩,王中铎
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