刘金磊,邓思平,江东能,陈华谱,李广丽,吴天利,田昌绪,朱春华

• 1:广东海洋大学水产学院//广东省名特优鱼类生殖调控与繁育工程技术研究中心
• 2:广东省水生经济动物良种繁育重点实验室//中山大学生命科学学院

摘要(Abstract)：

【目的】研究雌激素相关受体(Estrogen-related receptor,ERR)在雌性罗氏沼虾生殖过程中的功能。【方法】通过注射双链RNA(Double-strandedRNA,dsRNA)干扰罗氏沼虾(Macrobrachium rosenbergii)ERR的表达,对干扰组和对照组的卵巢样本进行转录组测序,筛选与生殖相关的差异表达基因,并通过qPCR检测它们在卵巢发育过程中的表达。【结果】注射ERR-dsRNA后,卵巢中ERR mRNA和ERR蛋白的表达显著下降。ERR-dsRNA干扰组和对照组卵巢转录组测序获得的差异表达Unigene中,9条被富集到生殖GO条目,3条被富集到生殖过程GO条目,21条被富集到卵母细胞减数分裂Pathway条目。这些生殖相关的Unigene中差异倍数较大的有9条,经qPCR验证,5条在ERR干扰组和对照组间的表达差异有统计学意义。这5条Unigene中有3条在卵巢发育过程中的表达差异有统计学意义,其中2条(CL7112.Contig4_All和Unigene4600_All)与卵母细胞减数分裂有关,1条(CL4888.Contig2_All)与生殖相关激素的合成及释放有关。【结论】ERR可通过调控卵母细胞减数分裂和生殖相关激素的合成与释放调控罗氏沼虾卵巢发育。

关键词(KeyWords)： 罗氏沼虾;雌激素相关受体(ERR);生殖;RNA干扰(RNAi);转录组分析

Abstract:

Keywords:

基金项目(Foundation): 广东省海洋与渔业科技推广专项(B201500B02;A201608B01);; 广东省自然科学基金(2016A030313743,2017A030313101);; 广东省水生经济动物良种繁育重点实验室(2018-2019年)开放基金课题

作者(Author): 刘金磊,邓思平,江东能,陈华谱,李广丽,吴天利,田昌绪,朱春华

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