董宏标,陈刚,张明辉,张健东,周晖,汤保贵,黄建盛,施刚,姜玲

• 1:广东海洋大学水产学院广东省南海水产经济动物增养殖重点实验室

摘要(Abstract)：

用聚丙烯酰胺垂直板不连续凝胶电泳法对湛江海域褐菖鲉(Sebastiscus marmoratus)心、肝、肾、性腺、肌肉、鳃、眼、脑等8种组织的12种同工酶(ADH、a-AMY、CAT、EST、GDH、LDH、MDH、ME、POD、PPO、SDH、SOD)进行分析。结果表明,12种同工酶均有明显的组织特异性。经筛选,对肝脏和眼的8种同工酶(ADH、EST、LDH、MDH、ME、POD、SDH、SOD)进行群体遗传结构分析,共记录了18个基因位点,其中呈多态性的基因位点有5个(Adh-1、Est-2、m-Pod-1、s-Pod-1、s-Sod-1),多态位点比例(P)为27.78%,平均有效等位基因数(Ae)为1.238 8,平均观测杂合度(Ho)值为0.1401。

关键词(KeyWords)： 褐菖鲉;同工酶;组织特异性;遗传结构

Abstract:

Keywords:

基金项目(Foundation): 广东省科技厅科技计划项目资助(2006B20201059)

作者(Author): 董宏标,陈刚,张明辉,张健东,周晖,汤保贵,黄建盛,施刚,姜玲

参考文献(References)：

• [1]吴常文.浙江舟山近海褐菖鲉Sebastiscus marmoratus生物学研究[J].浙江海洋学院学报(自然科学版),1999,18(3):185-190.
• [2]Fujita,H.and Kohda.M.Male mating effort in the viviparous scorpion fish,Sebastiscus marmoratus[J].Ichthyol.Res.,1996,43(3):247-255.
• [3]郑榕辉,王重刚,李岩.三丁基锡暴露对褐菖鲉碱性磷酸酶活性的影响[J].厦门大学学报(自然科学版),2005,44:203-206.
• [4]朱爱意,赵向炯,付俊.褐菖鲉耗氧率及窒息点的初步研究[J].海洋水产研究,2007,28(1):95-100.
• [5]Kita J,Tsuchida S,Setoguma T.Temperature preference and tolerance,and oxygen consumption of the marbled rockfish,Sebastiscus marmoratus[J].Marine Biology,1996,(125):467-471.
• [6]Wada S,Hanjavanit C,Kurata O,et al.Ochroconis humicola infection in red sea bream Pagrus major and marbled rockfish Sebastiscus marmoratus cultured in Japan[J].Fisheries Science,2005,(71):682-684.
• [7]Kohbara J,Oohara K,Matsuda T,et al.Gustatory receptor responses in marbled rockfish Sebastiscus marmoratus[J].Fisheries Science,2002,(68):862-871.
• [8]Masaya K,Muneharu T.Genetic and morphological identification of Sebastiscus tertius in the East China Sea(Scorpaeniformes:Scorpaenidae)[J].Ichthyol Res,2001,48:247-255.
• [9]王中仁.植物等位酶分析[M].北京:科学出版社,1998:95-151.
• [10]小川和郎,中根一穗.酶组织细胞化学技术[M].上海:上海医科大学出版社,1989:21-62.
• [11]Shaklee J B,Allendorf F W,Morrizot D C,et al.Gene nomenclature for protein coding loci in fish[J].Trans Am Fish Soc,1990,119:2-15.
• [12]Japan Fisheries Resource Conservation Association.Population differentiation of marine organism by isozyme analysis[R].Report on the genetic assessment project.Tokyo:Japan Fisheries Resource Conservation Association,1989:28-209.
• [13]Ren Y,Gao T,Yang T.Isozyme analysis on the populations of Ruditapes philippinarum[J].Journal of ocean university of China,2006,5(1):58-62.
• [14]戈志强,宋学宏,许爱国,等.红鲌属3种鲌鱼同工酶的比较研究[J].水利渔业,2005,25(6):23-24.
• [15]Shaklee J B,Beacham T D,Seeb L,et al.Managing fisheries using genetic data:Case studies from four species of pacific salmon[J].Fishery Research,1999,43(1):45-78.
• [16]林信伟,熊全沫.寡龄新银鱼同工酶及其与幼态持续的关系[J].遗传学报,1991,18(3):214-218.
• [17]邬勇,李明云,范华等.美国红鱼(Sciaenops ocellatus)鳍条组织的同工酶表达[J].宁波大学学报(理工版),2005,18(4):463-466.
• [18]李思发,吴力钊,王强,等.长江、珠江、黑龙江鲢、鳙、草鱼种质资源研究[M].上海:上海科学技术出版社,1990:83-101.
• [19]王可玲,张培军,刘兰英,等.中国近海带鱼生化遗传结构及其鉴别的研究[J].海洋学报,1994,(1):93-104.
• [20]Menezes M R,Taniguchi N,Seki S.Degree of intraspecific genetic divergence and variability in three sciaenid species[J].Japanese J Ichthyology,1990,37(1):39-48.
• [21]王伟继,孔杰,庄志猛,等.真鲷野生群体和人工繁殖群体的同工酶差异[J].生物多样性,2000,(4):391-396.
• [22]李明云,张海琪,竺俊全,等.象山港养殖大黄鱼同工酶的分析[J].海洋学报,2003,25(2):231-236.
• [23]Smith P J,Fujio Y.Genetic variation in marine teleosts:High variability in habitat specialists and low variability in habitat generalists[J].Marine biology,1982,69:7-20.