杨善,杨杰文,叶昌辉,莫俊杰,周鸿凯

• 1:广东海洋大学农学院

摘要(Abstract)：

为探究干旱胁迫下分蘖期甘蔗植株内游离脯氨酸积累及其合成关键酶的响应机制,以甘蔗品种ROC22为材料进行桶栽干旱胁迫实验,测定生长于7种不同土壤水分下的甘蔗叶片中丙二醛含量、电导率、游离脯氨酸含量以及δ-OAT、P5CS、SOD的活性。结果表明,随着干旱胁迫强度增大,植株体内不同程度地激活了P5CS、δ-OAT、SOD的活性,合成积累了大量的游离脯氨酸,以抵抗丙二醛等氧化物的积累所造成对细胞膜透性的破坏。但在沙土的土壤水分为2.64%(相对持水量21.2%)时,达到了ROC22的萎蔫系数。相关统计分析结果,6个甘蔗生理指标间的简单正相关均达到了显著水平。偏相关分析结果,细胞膜透性分别与P5CS活性、脯氨酸含量为负的偏相关且达到了显著水平,丙二醛、δ-OAT、SOD分别与脯氨酸含量的偏相关系数均到达了极显著水平。此外,P5CS与SOD存在共线性,多元相关性分析表明P5CS对游离脯氨酸积累的作用显著地大于δ-OAT,在干旱胁迫下,甘蔗脯氨酸的两个合成途径表现为以Glu→Pro途径主,Orn→Pro途径为辅。

关键词(KeyWords)： 甘蔗;脯氨酸;P5CS;δ-OAT;干旱胁迫

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41073059);; 广东省科技计划项目(2013B020301005,2013B020301008);; 广东省良种培育和引进专项(201201148)

作者(Author): 杨善,杨杰文,叶昌辉,莫俊杰,周鸿凯

参考文献(References)：

• [1]INES Slama,TAHAR Ghnaya,DORSAF Messedi,et al.Effect of sodium chloride on the response of the halophyte species Sesuvium portulacastrum grown in mannitol-induced water stress[J].J Plant Res,2007,120(2):291–299.
• [2]OUZOUNIDOU G,ILIAS I F,GIANNAKOULA A,et al.Effect of water stress and Na Cl triggered changes on yield,physiology,biochemistry of broad bean(Vicia faba)plants and on quality of harvested pods[J].Biologia,2014,69(8):1010-1017.
• [3]RUTHERFORD R S.The assessment of proline accumulation as a mechanism of drought resistance in sugarcane[J].Prec S Afric Sug Tech Asseci,1989,63:136-141.
• [4]李富生,何丽莲.植物对非生物胁迫的生理响应及甘蔗抗旱抗寒性研究进展[J].甘蔗,2004,11(1):31-37.
• [5]陈如凯,张木清,陆裔波.干旱胁迫对甘蔗生理影响的研究[J].甘蔗,1995,2(1):1-6.
• [6]杨建波,诸葛少军,黎海涛,等.干旱胁迫对甘蔗生长生理的影响及品种抗旱性评价[J].南方农业学报,2012,43(8):1114-1120.
• [7]黄诚梅,毕黎明,杨丽涛,等.聚乙二醇胁迫对甘蔗伸长期间叶中脯氨酸积累及其代谢关键酶活性的影响[J].植物生理学通讯,2007,43(1):77-80.
• [8]刘洋,姚艳丽,林希昊,张海林,苏俊波.干旱胁迫对甘蔗近缘材料抗氧化系统酶活性的影响[J].西南农业学报,2012,25(3):852-855.
• [9]高俊凤.植物生理学实验技术[M].西安:世界图书出版公司,2000.
• [10]周钟瑜.土壤水分测定方法[M].北京:水利水电出版社,1986:11-12.
• [11]KAVI KISHOR P B,HONG Z L,MIAO G H,et al.Overexpression of△1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants[J].Plant Physiol,1995,108(4):1387-1394.
• [12]黄诚梅.甘蔗脯氨酸积累与△1-吡咯啉-5-羧酸合成酶(Sc P5CS)基因克隆及转化研究[D].广西大学,2007:17-18.
• [13]HU C-AA,DELAUNEY A J,VERMA D P S.A bifunctional enzyme(△1-pyrroline-5-carboxylate synthetase)catalyzes the first two steps in proline biosynthesis in plants[J].Proc Natl Acad Sci USA 1992,89(19):9354-9358.
• [14]Kim H R,Rho H M W,Park J W,et al.Assay of ornithine aminotransferase with ninhydrin[J].Anal Biochem,1994,223(2):205-207.
• [15]BATES L S,WALDREN R P,TEARE I D.Rapid determination of free proline for water-stress studies[J].Plant and Soil,1973,39(1):205-207.
• [16]周鸿凯,叶振邦.干旱对甘蔗叶片的细胞透性及脯氨酸积累效应的研究初报[J].甘蔗糖业,1988(4):39-41.
• [17]陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学通报,1989,6(4):211-217.
• [18]高三基,罗俊,张华,等.甘蔗抗旱性生理生化鉴定指标[J].应用生态学报,2006,17(6):1051-1054.
• [19]毛才良,刘有良.盐胁迫大麦苗体内的Na+、K+分配与叶片耐盐量[J].南京农业大学学报,1990,13(3):32-36.
• [20]罗俊,张木清,林彦铨,等.甘蔗叶绿体荧光参数、MDA含量及膜透性与耐旱性的关系[J].福建农业大学学报,1999,28(3):257-262.
• [21]KIYOSUE T,YOSHIBA Y,YAMAGUCHI S K.A nuclear gene encoding mitochondrial proline dehy-drogenase,an enzyme involved in proline metabolism,is upregulated by proline but downregulated by dehydration in Arabidopsis thaliana[J].Plant Cell,1996,8(8):1323-1335.
• [22]LEHMANN S,FUNK D,Szabados L.Proline metabolism and transport in plant development[J].Amino Acids,2010,39(4):949-962.
• [23]DELAUNEY A J,HU C-AA,KISHOR P B K,et al.Cloning of ornithine-aminotransferase c DNA from Vigna aconitifolia by trans-comple-mentation in Escherichia coliand regulation of proline biosynthesis[J].J Biol Chem 1993,268(25):18673-18678.
• [24]HERVIEU F,LE D L,HUAUL T C.Contribution of ornithine aminotransferase to proline accumulation in Na Cl treated radish cotyledons[J].Plant Cell Environ,1995,18(2):205-210.
• [25]ROOSENS N H C J,THU T T,ISKANDAR H M.Isolation of ornithine-aminotransferase c DNA and effect of salt stress on its expression in Arabidopsis[J].Plant Physiol,1998,117(1):263-271.
• [26]赵福庚,孙诚,刘友良.盐胁迫激活大麦幼苗脯氨酸合成的鸟氨酸途径[J].植物学报,2001,43(1):36-40.
• [27]IGARASHI Y,YOSHIBA Y,TAKESHITA T.Molecular cloning and characterization of a c DNA encoding proline transporter in rice[J].Plant Cell Physiol,2000,41(6):750-756.
• [28]BRUGIEREA N,DUBOISB F,LIMAMIA A M.Glutamine synthetase in the phloem plays a major role in controlling proline production[J].Plant Cell,1999,11(10):1995-2012.