周艳,汪旭,王蕴博,徐海青,王仕举,窦世娟,杨明,李莉云,刘国振

• 1:河北农业大学生命科学学院

摘要(Abstract)：

【目的】建立可用于大规模培养的户外开放式微藻培养体系。【方法】设计一种微藻平面开放浅层培养(Flat, open and shallow, FOS,简称浮法)体系,以小球藻(Chlorella sorokiniana)为模式藻种,探讨温度、光照、pH值和培养基营养成分等因素在该培养体系中对小球藻生长的影响,开展900 L体系的户外培养试验。【结果】浮法体系主要由塑料袋和垫板组成,在该培养体系中的小球藻在20～40℃范围内均可生长,最适生长温度为30～35℃。小球藻生长有明显的照度依赖性,无光时几不生长,随着照度升高,生长速度加快,在较高的照度下表现有光饱和现象。以TAP为基础培养基,在碳源或氮源缺乏时小球藻几不生长。pH 7.5左右有利于小球藻生长。900L体系的户外培养试验的生物量(干物质得率)为0.15 g/(L·d)。【结论】在此新型户外平面开放浅层微藻培养体系中,温度、照度、pH值及营养成分等对小球藻的生长均有不同程度的影响。该体系有成本低、操作简便、容易控制、自然资源利用率高等特点,有大面积推广潜力。

关键词(KeyWords)： 微藻;小球藻;浮法培养;培养基;大规模;生物量

Abstract:

Keywords:

基金项目(Foundation): 河北农业大学引进人才科研专项(YJ201910)

作者(Author): 周艳,汪旭,王蕴博,徐海青,王仕举,窦世娟,杨明,李莉云,刘国振

参考文献(References)：

• [1]ABO B O,ODEY E A,BAKAYOKO M,et al.Microalgae to biofuels production:a review on cultivation,application and renewable energy[J].Reviews on Environmental Health,2019,34(1):91-99.
• [2]于殿江,施定基,何培民,等.微藻规模化培养研究进展[J].微生物学报,2021,61(2):333-345.
• [3]JIN H,ZHANG H,ZHOU Z W,et al.Ultrahigh-cell-density heterotrophic cultivation of the unicellular green microalga Scenedesmus acuminatus and application of the cells to photoautotrophic culture enhance biomass and lipid production[J].Biotechnology and Bioengineering,2020,117(1):96-108.
• [4]LíVANSKYK,DOUCHA J.An estimation method for CO2/O2 photosynthetic rates and for CO/O2 mass transfer coefficients in outdoor algal culture units[J].Archiv Für Hydrobiologie,Supplement Volumes,1994,74:125-140.
• [5]DOUCHA J,LíVANSKYK.Novel outdoor thin-layer high density micro algal culture system:Productivity and operational parameters[J].Archiv Für Hydrobiologie,Supplement Volumes,1995,76:129-147.
• [6]DOUCHA J,STRAKA F,LíVANSKYK.Utilization of flue gas for cultivation of microalgae (Chlorella sp.) in an outdoor open thin-layer photobioreactor[J].Journal of Applied Phycology,2005,17(5):403-412.
• [7]DOUCHA J,LíVANSKYK.Production of high-density Chlorella culture grown in fermenters[J].Journal of Applied Phycology,2012,24(1):35-43.
• [8]DOUCHA J,LíVANSKYK.Productivity,CO2/O2exchange and hydraulics in outdoor open high density microalgal (Chlorella sp.) photobioreactors operated in a Middle and Southern European climate[J].Journal of Applied Phycology,2006,18(6):811-826.
• [9]WILEY P,HARRIS L,REINSCH S,et al.Microalgae cultivation using offshore membrane enclosures for growing algae (OMEGA)[J].Journal of Sustainable Bioenergy Systems,2013,3(1):18-32.
• [10]ZHU C B,ZHU H,CHENG L Y,et al.Bicarbonate-based carbon capture and algal production system on ocean with floating inflatable-membrane photobioreactor[J].Journal of Applied Phycology,2018,30:875-885.
• [11]ZHU C B,ZHANG R L,CHENG L Y,et al.A recycling culture of Neochloris oleoabundans in a bicarbonate-based integrated carbon capture and algae production system with harvesting by auto-flocculation[J].Biotechnology for Biofuels,2018,11:204.
• [12]ZHU C B,HAN D S,LI Y H,et al.Cultivation of aquaculture feed Isochrysis zhangjiangensis in low-cost wave driven floating photobioreactor without aeration device[J].Bioresource Technology,2019,293:122018.
• [13]ZHU C B,ZHAI X Q,XI Y M,et al.Efficient CO2capture from the air for high microalgal biomass production by a bicarbonate Pool[J].Journal of CO2Utilization,2020,37:320-327.
• [14]ZHU C B,ZHAI X Q,WANG J H,et al.Large-scale cultivation of Spirulina in a floating horizontal photobioreactor without aeration or an agitation device[J].Applied Microbiology and Biotechnology,2018,102(20):8979-8987.
• [15]ZHU C B,ZHAI X Q,JIA J,et al.Seawater desalination concentrate for cultivation of Dunaliella salina with floating photobioreactor to produceβ-carotene[J].Algal Research,2018,35:319-324.
• [16]ZHANG Q H,WU X,XUE S Z,et al.Hydrodynamic characteristics and microalgae cultivation in a novel flat-plate photobioreactor[J].Biotechnology Progress,2013,29(1):127-134.
• [17]YAN C H,ZHANG Q H,XUE S Z,et al.A novel low-cost thin-film flat plate photobioreactor for microalgae cultivation[J].Biotechnology and Bioprocess Engineering,2016,21(1):103-109.
• [18]LI T T,ZHENG Y B,YU L,et al.High productivity cultivation of a heat-resistant microalga Chlorella sorokiniana for biofuel production[J].Bioresource Technology,2013,131:60-67.
• [19]BLANC G,DUNCAN G,AGARKOVA I,et al.The Chlorella variabilis NC64A genome reveals adaptation to photosymbiosis,coevolution with viruses,and cryptic sex[J].The Plant Cell,2010,22(9):2943-2955.
• [20]GAO C F,WANG Y,SHEN Y,et al.Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome,transcriptomes,and proteomes[J].BMC Genomics,2014,15(1):582.
• [21]KUMAR G,SHEKH A,JAKHU S,et al.Bioengineering of microalgae:recent advances,perspectives,and regulatory challenges for industrial application[J].Frontiers in Bioengineering and Biotechnology,2020,8:914.
• [22]GORMAN D S,LEVINE R P.Cytochrome f and plastocyanin:their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii[J].Proceedings of the National Academy of Sciences of USA,1965,54(6):1665-1669.
• [23]METSOVITI M N,PAPAPOLYMEROU G,KARAPANAGIOTIDIS I T,et al.Effect of light intensity and quality on growth rate and composition of Chlorella vulgaris[J].Plants,2019,9(1):31.
• [24]高静思,朱佳,董文艺.光照对我国常见藻类的影响机制及其应用[J].环境工程,2019,37(5):111-116.
• [25]ZHENG H L,WU X D,ZOU G Y,et al.Cultivation of Chlorella vulgaris in manure-free piggery wastewater with high-strength ammonium for nutrients removal and biomass production:Effect of ammonium concentration,carbon/nitrogen ratio and p H[J].Bioresource Technology,2019,273:203-211.
• [26]ZHENG Y B,LI T T,YU X C,et al.High-density fed-batch culture of a thermotolerant microalga Chlorella sorokiniana for biofuel production[J].Applied Energy,2013,108:281-287.
• [27]HE Q N,YANG H J,HU C X.Culture modes and financial evaluation of two oleaginous microalgae for biodiesel production in desert area with open raceway pond[J].Bioresource Technology,2016,218:571-579.