芋螺毒素G1.9的氧化折叠及活性Oxidative Folding and Activity of Conotoxin G1.9
黄翠燕,廖燕玲,高炳淼
摘要(Abstract)：
【目的】通过合成芋螺毒素线性肽G1.9并优化氧化折叠体系，测试其对昆虫细胞毒性，为新型多肽类杀虫剂的设计提供依据。【方法】采用固相多肽合成法（SPPS）制备含4个半胱氨酸的芋螺毒素线性肽G1.9并优化氧化折叠体系，采用一罐法氧化芋螺毒素G1.9并验证其生物活性。【结果与结论】采用SPPS法成功地合成了线性肽G1.9，优化获得芋螺毒素G1.9-G型异构体的最佳氧化折叠体系是在0.1 mol/L碳酸氢铵缓冲液（pH 8.0）中氧化36 h。MTT法实验表明G1.9-G能显著抑制昆虫细胞sf9的生长，为后续研究开发新型、安全和高效的多肽类生物杀虫剂奠定基础。
关键词(KeyWords)： 芋螺毒素;固相多肽合成法;氧化折叠;活性
基金项目(Foundation): 海南省重点研发计划（ZDYF2022SHFZ309）;; 海南省自然科学基金（820RC636）
作者(Author): 黄翠燕,廖燕玲,高炳淼
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