董晓慧,杨原志,郑石轩,周歧存,张其华

• 1:广东海洋大学水产学院
• 2:广东海洋大学水产学院
• 3:湛江粤海饲料有限公司
• 4:广东海洋大学水产学院

摘要(Abstract)：

为研究氨基酸螯合钴对凡纳滨对虾的营养效果,在凡纳滨对虾配合饲料中添加5、10、15 mg/kg的氯化钴和蛋氨酸钴。饲养8周,4周末(饲养中期)称重,8周末称重并采集对虾肝胰脏和肌肉,测定肝胰脏和肌肉中的钴含量。结果表明:钴的添加形式和钴水平不影响对虾0~4周增重率,15 mg/kg蛋氨酸钴显著提高了对虾0~8周增重率,且两种形式的钴之间差异显著(P<0.05)。51、0 mg/kg的蛋氨酸钴和5、101、5 mg/kg的氯化钴不影响8周末对虾的增重率。特定生长率、饲料系数和蛋白质效率的变化趋势与增重率类似。钴的添加形式和钴水平不影响肌肉中的钴含量,15 mg/kg的氯化钴组肝胰脏中钴含量显著高于对照组(P<0.05),其他各组间无显著差异;蛋氨酸钴各组肝胰脏中钴含量无显著差异;钴的添加形式不影响肝胰脏中钴含量。统计结果表明,促进生长方面,两种钴之间存在显著差异,蛋氨酸钴的营养效果好于氯化钴。饲料中蛋氨酸钴的添加量为15 mg/kg时,对虾生长最快,5 mg/kg时,可满足需要。

关键词(KeyWords)： 饲料添加剂;蛋氨酸钴;氯化钴;凡纳滨对虾;生长;沉积

Abstract:

Keywords:

基金项目(Foundation): 湛江市招标项目“环保型对虾饲料及抗病添加剂的研制”

作者(Author): 董晓慧,杨原志,郑石轩,周歧存,张其华

参考文献(References)：

• [1]徐建雄.蛋氨酸铁在猪营养中的应用研究[J].上海农学院学报,1993,11(1):17-21.
• [2]Ammerman C B,Luo X G.Iron-methionine complex and feedgrade ferrous sulfate as sources of dietary iron for chicks[J].Anim Sci,1996,74(Suppl.1):8.
• [3]Yang W Z.Effect of chromium supplementation of early lacta-tion performance of Hoistein cows[J]Can J Anim Sci,1996,76:225-230.
• [4]Kashiwada K,Teshima S,Kanazawa A.Studies on the pro-duction of B vitamins by intestinal bacteria of fish-V.Evidenceof the production of vitamin B12by microorganisms in the in-testinal canal of carpCyprinus carpio[J].Nippon suisan gak-kaishi,1970,36:421-424.
• [5]Cunningham L W,Fischer R L,Westling C S.The binding ofzinc and cobalt by insulin[J].J Am Chem Soc,1955,77:5703-5707.
• [6]Roginski E E,Mertz W.A biphasic response of rats to cobalt[J].J Nutr,1977,107:1537-1542.
• [7]Halver J E.Utilization of ascorbic acid in fish[J].Ann N YAcad Sci,1975,268:81-102.
• [8]Halver J E.Nutrition of salmonid fishes.Ⅲ.Water-solublevitamin requirements of Chinook salmon[J].J Nutr,1957,62:225-243.
• [9]Sukhoverlov F M.Estimation of standing crops and rates offeeding in ponds[J].FAO Fish Rep,1967,44(3):416-423.
• [10]Khan H A,Mukhopadhay S K.Observation on the effects ofyeast and cobalt chloride in increasing the survival rate of thehatchlings ofHeteropneustes fossilis(Bloch)[C]//Symposi-umon trends of researchin zoology.Calcutta:Zoological socie-ty,1971:11-12.
• [11]魏万权,李爱杰.牙鲆幼鱼饲料中锰、钴适宜添加量的初步研究[J].浙江海洋学院学报(自然科学版),2001,20:83-87.
• [12]梁德海,刘发义,孙凤,等.中国对虾对Co需要量的研究[J].海洋科学,1991(3):12-14.
• [13]Anadu DI,Anozie O C,Anthony A D.Growth responses ofTilapia zilliifed diets containing various levels of ascorbicacid and cobalt chloride[J].Aquaculture,1990,88:329-336.
• [14]Paripatananont T,Richard T Lovell.Chelated zinc reduces thedietary zinc requirement of channel catfish[J].Aquaculture,1995,133:73-82.
• [15]Tan B P,Mai KS.Zinc methionine and zinc sulfate as sourcesof dietary zinc for juvenile abaloneHaliotis discus hannaiIno[J].Aquaculture,2001,192:67-84.
• [16]Kawashi ma T,Henry P R,Ammerman C B.Bioavailability ofcobalt sources for ruminants.2.Esti mation of the relativevalue of reagent grade and feed grade cobalt sources fromtis-sue cobalt accumulation and vitamin B12concentrations[J].Nutrition research,1997,17(6):957-974.
• [17]Henry P R,Littell R C,Ammerman C B.Bioavailability ofcobalt sources for ruminants.1.Effect of ti me and dietary co-balt concentration on tissue cobalt concentration[J].Nutri-tion research,1997,17(6):947-955.
• [18]刘发义,梁德海,孙凤,等.饵料中的铜对中国对虾的影响[J].海洋与湖沼,1990,21(5):404-410.
• [19]郭志勋,陈毕兰,徐力文,等.饲料中铜的添加量对凡纳滨对虾生长、血液免疫因子及组织铜的影响[J].中国水产科学,2003,10(6):526-528.
• [20]王维娜,王安利,刘存岐,等.饵料的铜含量对中国对虾生长及其体内铜、锌和铁含量的影响[J].水产学报,1997(3):258-262.