郝甜甜,于朝磊,孙永智,李宝山,王晓艳,王成强,李璐,黄炳山

• 1:山东省海洋资源与环境研究院/山东省海水渔用饲料工程技术研究中心/水生动物营养与饲料研发创新示范平台/山东省海洋生态修复重点实验室/烟台市海珍品质量安全控制与精深加工重点实验室
• 2:烟台经海海洋渔业有限公司

摘要(Abstract)：

【目的】探究饲料中添加胍基乙酸（GAA）对许氏平鲉（Sebastes schlegelii）生长性能、消化吸收、代谢及抗氧化能力的影响，为GAA在许氏平鲉网箱养殖配合饲料中的应用提供依据。【方法】取初始体质量为（125.00±1.00）g许氏平鲉，分别投喂添加质量分数0.00%、0.01%、0.02%、0.04%、0.08%的胍基乙酸5种等氮等脂饲料（分别记为D0、D1、D2、D3和D4组）7周，测定许氏平鲉生长性能、消化酶活力、抗氧化能力、血清生化指标和肝脏糖代谢等指标。【结果】1）与对照组（D0）相比，D1、D2、D3和D4组显著提高了鱼体增重率、特定生长率和蛋白质效率，降低了饲料系数（P <0.05）;2）饲料中添加胍基乙酸对全鱼水分、粗蛋白、粗脂肪、粗灰分均无显著影响（P> 0.05）;3）与D0组相比，D1、D2、D3和D4组肠道胰蛋白酶和淀粉酶活力显著升高（P <0.05），脂肪酶活力则在D3和D4组显著升高（P <0.05）;4）与D0组相比，D3组血清甘油三酯和高密度脂蛋白胆固醇含量显著升高（P <0.05）;5）与D0组相比，D1、D2、D3和D4组肝脏总抗氧化能力显著提高（P <0.05），丙二醛含量则在D1、D2、D3和D4组显著降低（P <0.05）,D2、D3和D4组超氧化物歧化酶活力显著提高（P <0.05）。6）与D0组相比，D1、D2、D3和D4组肝脏丙酮酸激酶活力和肌酸激酶活力均显著降低（P <0.05），磷酸果糖激酶活力则在D3组显著降低（P <0.05）,D2、D3和D4组肝脏中肌酸（Cr）含量显著升高（P <0.05）。【结论】以增重率为评价指标，许氏平鲉饲料中胍基乙酸的适宜添加量为0.04%；饲料中添加0.04%～0.08%胍基乙酸可提高许氏平鲉肠道消化酶活力，并可通过调节代谢产物Cr的含量发挥抗氧化和能量调节作用。

关键词(KeyWords)： 胍基乙酸;许氏平鲉;生长性能;消化吸收;代谢;抗氧化能力

Abstract:

Keywords:

基金项目(Foundation): 山东省重点研发计划（科技示范工程）（2021SFGC0701）;; 山东省自然科学基金青年基金（ZR2020QC206）

作者(Author): 郝甜甜,于朝磊,孙永智,李宝山,王晓艳,王成强,李璐,黄炳山

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