李莉萍,陈明,罗永巨,唐章生,唐瞻杨,陈忠,张永德,曾兰,林勇

• 1:广西水产研究所

摘要(Abstract)：

采用RACE技术,对两个奥利亚罗非鱼群体(Oreochromis aurea)(美国奥利亚和中国奥利亚)热休克蛋白Hsp70基因完整编码区(code sequences,CDS)cDNA的进行克隆测序。序列分析结果表明:两个奥利亚罗非鱼群体热休克蛋白Hsp70基因CDS序列完全相同,全长1 923 bp,编码640个氨基酸,相对分子质量为70.29×103,理论等电点5.462,均具有Hsp70家族的3个签名序列:IDLGTTYS、IFDLGGGTFD、VVLVGGSTRIPKIQK;核定位信号标签KRKHKKDISQNKRALRR;Dank特征基序DLGTT-S-V;胞质Hsp70特征基序EEVD;靠近C端的GGMP4肽序列;2个糖基化位点NKSI和NVSA。对所得基因序列与已发表的青锵(Oryzias latipes)等物种Hsp70基因的氨基酸序列进行同源性比较,发现两个奥利亚罗非鱼群体与莫桑比克罗非鱼最高99.4%,与牙鲆最低83.9%;系统进化树分析表明奥利亚罗非鱼的Hsp70 cDNA序列与青鳉等物种的Hsp70基因聚在一支,而与牙鲆的Hsp70基因相分离。

关键词(KeyWords)： 奥利亚罗非鱼;热休克蛋白70(Hsp70)基因;cDNA;克隆;序列分析

Abstract:

Keywords:

基金项目(Foundation): 2008年广西壮族自治区直属公益性科研院所基本科研业务费专项资金项目(2060302GXIF-2008-04)

作者(Author): 李莉萍,陈明,罗永巨,唐章生,唐瞻杨,陈忠,张永德,曾兰,林勇

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