高山榕叶斑病菌的分离及鉴定Pathogen Isolation and Identification of Leaf Spot Disease of Ficus altissima
章月琴,成嘉欣,邹奕园,胡汉桥
摘要(Abstract):
从高山榕(Ficus altissima Bl.)叶斑病的病叶上分离到一株真菌,经接种鉴定为高山榕叶斑病的病原物。通过形态学观察,发现该病原物为链格孢属(Alternaria sp.)。利用直接菌落PCR测定了此病原物的核糖体DNA的转录间区、内聚半乳糖醛酸酶基因以及基因组中OPA1-3区域的DNA。结合序列分析和形态学观察结果,该菌鉴定为A.gaisen。
关键词(KeyWords): 高山榕;叶斑病;链格孢;分子鉴定
基金项目(Foundation): 广东省科技攻关项目(2013B020309010)
作者(Author): 章月琴,成嘉欣,邹奕园,胡汉桥
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