黄丹,刘东超,王晓梁,林挺,黄晓健,欧阳兆攀

• 1:广东海洋大学水产学院
• 2:广东省藻类养殖与应用工程技术研究中心

摘要(Abstract)：

【目的】阐明长茎葡萄蕨藻对无机碳的利用方式。【方法】运用pH漂移技术,探索藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻(Caulerpa lentillifera)的pH补偿点的影响,并研究在3种不同温度(20、25、30℃)下,乙酰唑胺(AZ)、乙氧苯丙噻唑磺胺(EZ)、4,4′-二异硫氰基芪2,2′-二磺酸(DIDS)、钒酸盐(Van)4种抑制剂对长茎葡萄蕨藻无机碳的利用影响。【结果】藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻的pH补偿点均无显著性影响(P>0.05),在一定范围内藻体质量或照度越大,达到pH补偿点时间越短,且pH补偿点均大于9。20℃下各抑制剂的抑制作用最大,其次为30℃,25℃最小,但3种温度下各抑制剂的抑制率无显著差别(P> 0.05)。4种抑制剂中DIDS对长茎葡萄蕨藻pH补偿点和无机碳利用均无显著影响(P> 0.05),剩余3种抑制剂的抑制作用依次为EZ>AZ>Van。【结论】藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻pH补偿点无影响。温度对长茎葡萄蕨藻吸收利用无机碳没有明显影响。长茎葡萄蕨藻能利用HCO_3~-作为无机碳源进行光合作用。

关键词(KeyWords)： 长茎葡萄蕨藻;pH补偿点;无机碳利用;温度;抑制剂

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(2015A020209167)

作者(Author): 黄丹,刘东超,王晓梁,林挺,黄晓健,欧阳兆攀

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