范德朋,胡亚冬,杨敏志,雷明科,王琦

• 1:碧沃丰生物科技(广东)股份有限公司
• 2:碧沃丰工程有限公司
• 3:广东海洋大学水产学院

摘要(Abstract)：

【目的】鉴定一株鱼腥藻藻华水体的溶藻菌,分析菌株的溶藻特性。【方法】从佛山近郊湖泊鱼腥藻水华水体中分离到一株溶藻菌BWFA55,通过菌株生理生化鉴定和16S rDNA基因序列分析鉴定菌种。将该菌株培养4 d,分析菌株的生长特征和溶藻活性。将BWFA55发酵上清液与水华鱼腥藻(Anabaena flos-aquae)共培养168 h,通过扫描电镜观察藻细胞形态。将BWFA55菌悬液接种至不同浓度的水华鱼腥藻(A. flos-aquae)藻液,另按不同接种量将BWFA55接种至同浓度藻液,分析藻液浓度及接种量对溶藻效果的影响。测定BWFA55无细胞上清液、菌体悬液、菌株原发酵液的溶菌活性,分析BWFA55的主要溶藻方式。采用透析和有机溶剂萃取分离溶藻物质,在不同温度、p H条件下研究溶藻活性物质的特性。【结果与结论】经鉴定,溶藻菌株BWFA55为地衣芽孢杆菌(Bacillus licheniformis)。该菌株24 h发酵液的溶藻活性最高,在接种量(体积分数)为10%的条件下,水体中水华鱼腥藻的叶绿素a(Chl-a)含量在7 d内下降80%以上。该菌通过分泌溶藻化合物间接攻击水华鱼腥藻,使丝状群体的藻细胞数量明显降低,群体连接结构被破坏。BWFA55主要的溶藻活性物质存在于发酵上清液中,分子质量大于3 ku,且可被乙酸乙酯萃取,在高温下保持60%以上的溶藻率,且不受p H影响,有良好的酸碱、温度耐受性。

关键词(KeyWords)： 水华;水华鱼腥藻;芽孢杆菌;溶藻细菌

Abstract:

Keywords:

基金项目(Foundation): 2019年佛山市自筹经费类科技计划项目（公共服务能力提升项目）（1920001001601）;; 国家重点研发计划（2019YFD0900200）;; 广东省企业科技特派员项目（GDKTP2020017800）

作者(Author): 范德朋,胡亚冬,杨敏志,雷明科,王琦

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