张维柱,余招龙,艾伟岭

• 1:滁州学院化学与生命科学系
• 2:电白冠利达海洋生物科技养殖有限公司

摘要(Abstract)：

采集采自山东某养殖场的栉孔扇贝(Chlamys farreri)健康贝和病贝的血淋巴,测定其血浆蛋白浓度、血浆蛋白图谱和相对分子质量,以及血浆抗菌活性、凝集素活性等几种重要的体液免疫因素,研究环境胁迫对血浆蛋白及其抗菌活性的影响。结果表明:栉孔扇贝(C.farreri)血浆蛋白质量浓度约1 260~1 650μg/mL;血浆蛋白SDS-PAGE图谱显示3条蛋白带,相对分子质量分别为34 000、25 000和14 000;健康贝血浆抗菌活性指数BI为0.34,病贝BI为0.21;凝集素活力水平为1.602~2.204。氨氮胁迫下血浆蛋白浓度下降约780μg/mL,凝集素活力下降为1.301;高温胁迫下血浆蛋白质量浓度下降约为900μg/mL,凝集素活力为1.903;高盐胁迫下血浆蛋白浓度下降约为990μg/mL,凝集素活力为2.204。

关键词(KeyWords)： 栉孔扇贝;血浆蛋白浓度;抗菌活性;凝集活性;SDS-PAGE

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30170741)

作者(Author): 张维柱,余招龙,艾伟岭

参考文献(References)：

• [1]Cromin M A,Culloty S C,Mulcahy M F.Lysozyme activity and protein concentration in the haemolymph of the flat oyster Ostrea edulis[J].Fish&Shellfish Immunology,2001,11(7):611-622.
• [2]Culloty S C,Mulcahy M F.Season-,age-and sex-related variation in the prevalence of bonamiasis in flat oysters(Ostrea edulis L.)on the south coast of Ireland[J].Aquaculture,1991,144(1-3):53-63.
• [3]Camino Ordása M,Amando Ordásb,Carmen Belosoa,et al.Immune parameters in carpet-shell clams naturally infected with Perkinsus atlanticus[J].Fish&Shellfish Immunology,2000,10(7):597-609.
• [4]Carballal M J,Lopez C,Azevedo C,et al.Enzymes involved in defense functions of hemocytes of mussel Mytilus galloprovincialis[J].J Invert Pathol,1997,70(2):96-105.
• [5]孙虎山,李光友.大肠杆菌感染后栉孔扇贝血淋巴中7种酶活性的变化[J].海洋科学,1999(5):40-44.
• [6]Xue Q G.,Renault T.Enzymatic activities in European flat oyster,Ostrea edulis,and Pacific oyster,Crassostrea gigas,hemolymph[J].J Invertebr Pathol,2000,76:155-163.
• [7]Luna-González A,Maeda-Martínez AN,Vargas-Albores F,et al.Phenoloxidase activity in larval and juvenile homogenates and adult plasma and haemocytes of bivalve mollusks[J].Fish&Shellfish Immunology,2003,15(4):275-282.
• [8]Zhang Weizhu,Wu Xinzhong,Sun Jingfeng,et al.Morphological and functional characterization of the haemocytes of the scallop,Chlamys farreri[J].Journal of Shellfish Research,2005,24(4):931-933.
• [9]张维翥,吴信忠,李登峰,等.栉孔扇贝血液细胞的免疫功能[J].动物学报,2005,51(4):669-677.
• [10]张维翥,吴信忠,李登峰,等.栉孔扇贝大量死亡的流行病学调查研究[J].海洋学报,2006,28(5):90-100.
• [11]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72(1-2):248-254.
• [12]Laemmli U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J].Nature,1970,227(15):680-685.
• [13]莫照兰,李会荣,俞勇.细菌糖蛋白对螯虾免疫因子的影响[J].中国水产科学,2000,7(3):28-32.
• [14]Perazzolo L M,Rogerio Gargioni,Paulo Ogliari,et al.Evaluation of some hemato-immunological parameters in the shrimp Farfantepenaeus paulensis submitted to environmental and physiological stress[J].Aquaculture,2002,214(1-4):19-33.
• [15]Renwrantz L,Stahmer A.Opsonizing properties of an isolated hemolymph agglutinin and demonstration of lectin-like recognition molecules at the surface of hemocytes from Mytilus edulis[J].J Comp Physiol,1983,149(4):535-546.