陈刚,欧光海,温震威,黄鉴鹏,马骞,张健东,谢瑞涛,李豫,蒋鑫涛,黄建盛

• 1:广东海洋大学水产学院
• 2:广东恒兴饲料实业股份有限公司

摘要(Abstract)：

【目的】探究饲料中添加水飞蓟素对杂交石斑鱼（Epinephelus fuscoguttatus♀×Epinephelus polyphekadion♂）幼鱼血清生化指标、脂代谢相关基因的表达以及肝脏代谢组的影响，为水飞蓟素在杂交石斑鱼饲料中开发利用提供参考。【方法】取（24.06±0.15）g杂交石斑鱼幼鱼，分别投喂添加0、0.2、0.4和0.6 g/kg的水飞蓟素4种等氮等脂饲料（分别记为SL0、S1、S2和S3）56 d，测定石斑鱼的血清生化指标和脂代谢相关基因的表达，对肝脏进行代谢组分析。【结果】1）与对照（SL0组）相比，S1和S2组血清中甘油三酯的含量显著下降，S3组血清中总胆固醇、低密度脂蛋白胆固醇、谷草转氨酶和谷丙转氨酶的含量显著下降，S2组血清中总蛋白、白蛋白和高密度脂蛋白胆固醇的含量显著上升（P <0.05）;2）与SL0组相比，S1、S2和S3组肝脏中hmgcr的表达水平显著下调以及pparγ的表达水平显著上调，S2组肝脏中pparα的表达水平显著上调（P <0.05）;3）与SL0组相比，S1和S2组肌肉中pparγ的表达水平显著下调（P <0.05）;4）与SL0组相比，在S3组肝脏代谢组中筛选出18种差异代谢物，其中共有15种差异代谢物下调，3种差异代谢物上调；谷胱甘肽代谢和鞘脂信号通路两条代谢通路被显著富集，5-羟脯氨酸、腺苷和牛磺鹅去氧胆酸等代谢物发生显著变化。【结论】饲料中添加0.6 g/kg的水飞蓟素可通过调节脂质代谢相关基因的表达抑制肝脏胆固醇的合成，调节代谢物的含量发挥抗氧化和免疫调节作用，从而降低杂交石斑鱼幼鱼的血脂水平，改善肝脏健康。

关键词(KeyWords)： 水飞蓟素;杂交石斑鱼;棕点石斑鱼;清水石斑鱼;血清生化指标;脂代谢相关基因;代谢组

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划省部联动专项（2022YFD2401203）;国家重点研发计划蓝色粮仓科技创新重点专项（2020YFD0900204）

作者(Author): 陈刚,欧光海,温震威,黄鉴鹏,马骞,张健东,谢瑞涛,李豫,蒋鑫涛,黄建盛

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