陈惠萍,梁桉婕,吕冉晖,陈鸿,卢盛佳,薛佳,缪小兰,段杉

• 1:华南农业大学食品学院/广东省功能食品活性物重点实验室/广东省天然活性物工程技术研究中心

摘要(Abstract)：

【目的】分析罗非鱼（Oreochromis mossambicus）中优势耐冰温假单胞菌（Pseudomonas）特有的基因特征和耐冰温基因。【方法】采用全长16S rDNA测序找出冰温贮藏罗非鱼中的优势假单胞菌，进行分离、鉴定；通过Illumina平台对其进行基因组从头测序，并通过基因家族分析确定耐冰温假单胞菌的核心基因家族，再与不耐冰温的铜绿假单胞菌（Pseudomonas aeruginosa PAO1）的基因组比较，筛选出耐冰温假单胞菌特有的核心基因家族；结合文献报道的假单胞菌的寒冷适应基因筛查出耐冰温假单胞菌潜在的耐冰温基因。【结果与结论】发现并分离出冰温贮藏罗非鱼中丰度最高的5株腐败菌，经鉴定均为布氏假单胞菌（Pseudomonas bubulae）和莓实假单胞菌（Pseudomonas fragi）。这5株假单胞菌的核心基因家族有3 692个，其中861个在不耐冰温的铜绿假单胞菌的基因组中不存在，此861个特有的核心基因家族在群体感应、类固醇的降解、某些氨基酸和肽的代谢、脂肪酸降解、抗坏血酸和醛糖酸代谢、柠檬烯和蒎烯降解、丁酸代谢、苯乙烯降解和香叶醇降解等途径中显著富集。进一步筛查发现上述5株耐冰温假单胞菌带有不耐冰温的铜绿假单胞菌没有的des、cbpM、ousA、treP、treR、trxC和grX等基因。

关键词(KeyWords)： 罗非鱼;冰温贮藏;假单胞菌;鉴定;基因组测序;耐冰温基因

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金（2021A1515010590）

作者(Author): 陈惠萍,梁桉婕,吕冉晖,陈鸿,卢盛佳,薛佳,缪小兰,段杉

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