郭昱嵩,王中铎,谢子强,刘楚吾

• 1:广东海洋大学水产学院南海水产经济动物增养殖广东普通高校重点实验室

摘要(Abstract)：

以三亚外海的红鳍笛鲷(Lutjanus erythropterus)自然群体为研究对象,利用磁珠富集法开发19个适用于红鳍笛鲷的微卫星DNA标记,其中16个为多态性标记,等位基因数为2～11,期望杂合度为0.242～0.896,观测杂合度为0.156～1.000,Ler 14、Ler 29、Ler 49等3个位点偏离哈迪-温伯格平衡(P<0.0031),Ler9-Ler18、Ler12-Ler19、Ler46-Ler47、Ler46-Ler49、Ler18-Ler50、Ler14-Ler51等6对位点间检测到明显的连锁(P<0.05),Ler29、Ler13、Ler49、Ler19、Ler24、Ler25、Ler14、Ler51、Ler18、Ler9等10个位点属于高度多态位点[多态信息含量(PIC)>0.5],Ler47、Ler12、Ler15、Ler46等4个为中度多态位点(0.25>PIC>0.5)。

关键词(KeyWords)： 红鳍笛鲷;微卫星DNA;磁珠富集;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 国家“十一五”科技支撑计划项目(2007BAD29B03);; 国家自然科学基金(30972253);; 广东省自然科学基金(8452408801001272,10452408801004224)

作者(Author): 郭昱嵩,王中铎,谢子强,刘楚吾

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