马氏珠母贝养殖群体早期生长阶段的遗传多样性与有效亲本数量估计Genetic Diversity and Effective Population Size Estimate in the Stock of Pearl Oyster Pinctada fucata martensii at Early Growth Stages
李俊辉,刘青云,杜晓东,邓岳文,王庆恒
摘要(Abstract):
从大亚湾野生群体选择亲本繁育1个养殖群体,受精第6天、23天与80天分别进行优选,按50%选留比率选留快生长个体,分别在受精后第3天、30天120天取样,利用8对SSR标记分析养殖群体遗传多样性与估计有效亲本数量。结果表明,在3个生长阶段每个位点的平均等位基因数分别为3.750、3.875和3.625,平均观察杂合度分别为0.324 2、0.527 3和0.372 4,平均期望杂合度分别为0.551 1、0.561 3和0.504 8。有效亲本数量的估计分别为38、28和30,3个生长阶段估算的有效亲本数量均比实际所用到的亲本数量少。选优管养措施导致养殖群体的遗传多样性与有效亲本数量降低。
关键词(KeyWords): 马氏珠母贝;遗传多样性;有效群体数量;SSR标记
基金项目(Foundation): 广东省海洋与渔业局科技专项(A201308A10,Z2014009);; 广东省科技专项(2015A030302079)
作者(Author): 李俊辉,刘青云,杜晓东,邓岳文,王庆恒
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