王凡,刘飞,池梦

• 1:洛阳师范学院

摘要(Abstract)：

【目的】探讨三氯生（Triclosan,TCS）对斑马鱼早期发育阶段的性别干扰机制。【方法】将斑马鱼胚胎暴露于不同质量浓度的TCS(0、2、10、50μg/L)中至受精后50 d，采用HE染色、实时荧光定量PCR和酶联免疫技术分析斑马鱼幼鱼的性别比、下丘脑-垂体-性腺（HPG）轴相关基因表达以及性激素和卵黄蛋白原（Vtg）含量。【结果】与对照组比较，2μg/L和10μg/L组斑马鱼幼鱼雌性比例升高，50μg/L组性比不变，但出现雌雄间体。2μg/L TCS使促性腺激素释放激素2基因（gnrh2）、雌激素受体α基因（erα）、促卵泡激素受体基因（fshr）极显著上调（P<0.01），促性腺激素释放激素3(gnrh3)、脑型芳香化酶基因（cyp19b）、促卵泡激素β基因（fshβ）显著上调（P<0.05）;10μg/L TCS使雌激素受体β基因（erβ）、fshβ和黄体生成素受体基因（lhr）极显著上调（P <0.01）,gnrh3、cyp19b、erα、fshr显著上调（P<0.05）;50μg/L TCS使gnrh3显著上调（P<0.05）,cyp19b、fshr和lhr极显著上调（P<0.01）。各TCS处理组Vtg含量显著升高（P <0.05）;2μg/L组雌二醇（E2）和11-酮基睾酮（11KT）含量极显著降低（P<0.01）;10μg/L TCS组睾酮（T）显著降低（P <0.05）,11KT/E2极显著降低（P <0.01）;50μg/L组T极显著降低（P <0.01）,11KT/E2显著降低（P<0.05）。【结论】TCS影响斑马鱼早期阶段的雌性比例以及HPG轴相关基因表达，有雌激素效应，但这种性别分化干扰效应无明显的剂量依赖关系；斑马鱼雌性化与TCS的雌激素效应有关（E2/11KT和Vtg升高），而该效应的产生主要通过对HPG轴相关基因的表达调控来实现。

关键词(KeyWords)： 三氯生;斑马鱼;性别分化;下丘脑-垂体-性腺轴

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（31971524）;; NSFC-河南人才培养联合基金(U1504303)

作者(Author): 王凡,刘飞,池梦

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