草鱼种质退化相关SRAP分子标记的筛选Isolation, Cloning and Sequencing for SRAP Markers Related to Germ Degeneration in Cienoyharyngodoni dellus
丁炜东,曹丽萍,曹哲明
摘要(Abstract)：
采用相关序列扩增多态性(sequence-realted amplified polymorphism,SRAP)技术分析野生草鱼和家养草鱼,并筛选与草鱼种质退化相关的分子遗传标记。共进行88对引物组合的检测,产生标记数目共计905个。依据标记在群体中出现的频率和变化规律,共筛选出21个可能与种质相关的特异性标记,对这些特异性标记进行测序并将测序结果进行BLAST分析,发现测得片段中有8个片段能在GeneBank中找到同源性较高的序列,而其他片段与数据库中序列的相似性较低。
关键词(KeyWords)： 草鱼;种质退化;克隆;测序;SRAP分子标记
基金项目(Foundation): 国家自然科学基金资助项目(30471343)
作者(Author): 丁炜东,曹丽萍,曹哲明
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