王思盼,陈柏安,黄永健,林坤,崔建军,刘东超

• 1:广东海洋大学水产学院
• 2:广东省藻类养殖及应用工程技术中心

摘要(Abstract)：

【目的】筛选长茎葡萄蕨藻（Caulerpa lentillifera）益藻细菌并评价其促生长性能，验证菌株对藻体的促生长效果。【方法】通过16S rDNA测序和平板涂布法对养殖长茎葡萄蕨藻进行藻际细菌菌群多样性分析、藻际可培养细菌分离鉴定及其促生特性分析；将获得的菌株制成菌悬液，与长茎葡萄蕨藻培养液按照体积比1∶400混合构建藻菌共生功能体，进一步验证菌株对藻体的促生效果。【结果】长茎葡萄蕨藻的附生细菌在门水平上以变形菌门和拟杆菌门为主要优势类群，分别占总菌的46.0%和41.2%；通过平板涂布法分离培养共获得9株细菌菌株，经鉴定属于8个属，分别为Formosa属、假鲁杰氏菌属（Pseudoruegeria）、短杆菌属（Brevibacterium）、深海单胞菌属（Thalassomonas）、Tenacibaculum属、鲁杰氏菌属（Ruggeria），弧菌属（Vibrio）和芽孢杆菌属（Bacillus），其中6株具有溶解有机磷能力的菌株分属于Formosa属、假鲁杰氏菌属、短杆菌属、鲁杰氏菌属和弧菌属，5株具有溶解无机磷能力的菌株分属于Formosa属、短杆菌属、深海单胞菌属和芽孢杆菌属，3株具有固氮能力的菌株分属于假鲁杰氏菌属、Tenacibaculum属和弧菌属，1株具有产吲哚-3-乙酸（IAA）能力和1株可以生产铁载体的菌株依次分属于弧菌属和深海单胞菌属。在藻菌共培养中，菌株Cl-9菌悬液可极显著地促进长茎葡萄蕨藻的生长（P <0.01）。【结论】筛选出一株长茎葡萄蕨藻益藻细菌（Bacillus sp. Cl-9），该菌可以与长茎葡萄蕨藻构建稳定的共生体系，显著提高藻体的生长，具有应用于工厂化养殖的潜力。

关键词(KeyWords)： 长茎葡萄蕨藻;藻菌共生;藻际菌群;促生菌

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划（2015A020209167）

作者(Author): 王思盼,陈柏安,黄永健,林坤,崔建军,刘东超

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