林明德,陈刚,马骞,王忠良,张健东,谢瑞涛,汤保贵,黄建盛,周晖,施钢,潘传豪,ERIC Amenyogbe,邓文鑫

• 1:广东海洋大学水产学院
• 2:广东恒兴饲料实业股份有限公司
• 3:广东粤海饲料集团公司

摘要(Abstract)：

【目的】筛选褐点石斑鱼(Epinephelus fuscoguttatus)及其杂交子一代(E. fuscoguttatus♀×E. polyphekadion♂,F1)的差异表达基因,探讨褐点石斑鱼及F1代之间的生长差异性。【方法】采用Illumina HiSeq 2000测序平台对同龄的褐点石斑鱼及其杂交F1的脑、肝脏和肌肉组织进行转录组测序,通过edgeR软件包筛选差异表达基因,并对差异表达基因进行GO(Gene Ontology)功能注释和KOG注释,最后通过实时荧光定量PCR验证转录组数据。【结果】测序的原始数据经过质量控制和组装共得到54 610条unigenes。组装的unigenes共有28 832条unigenes得到注释,共获得20234条差异表达基因,其中有10218条上调,10016条下调。GO功能注释显示共34061条unigenes聚类到涉及生物过程、分子功能和细胞组分的53个功能类别,其中被较多基因聚类到的功能类别为细胞过程、结合、单一生物过程、代谢过程和催化活性等。KOG注释发现,42 753 unigenes被注释到25个功能类别中,其中较多涉及信号转导机制、基因功能预测、翻译后修饰,蛋白质周转和分子伴侣、转录等。进一步筛选到一批和生长相关的差异表达基因,如GH、GHRH、PRL、SLP、AVTp等,这些差异表达基因在F1中的表达水平均高于其亲本褐点石斑鱼,与杂交F1相较于褐点石斑鱼生长快速的性状相符。【结论】研究完成了对褐点石斑鱼及其杂交F1的转录组测序、组装,获得了测序数据在不同数据库的功能注释信息,同时筛选了一批生长相关差异表达基因,这些基因在F1中的表达水平均高于其亲本褐点石斑鱼,与现实生长性状相符。

关键词(KeyWords)： 褐点石斑鱼;杂交石斑鱼;转录组测序;比较分析;生长相关基因

Abstract:

Keywords:

基金项目(Foundation): 2016广东省省级科技计划项目“石斑鱼杂交新品种选育”(2016B020201009)

作者(Author): 林明德,陈刚,马骞,王忠良,张健东,谢瑞涛,汤保贵,黄建盛,周晖,施钢,潘传豪,ERIC Amenyogbe,邓文鑫

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