李二超,陈立侨,蔡永久,顾顺樟,洪美玲,张璐,刘超

• 1:华东师范大学生命科学学院
• 2:华东师范大学生命科学学院
• 3:美国ADM公司
• 4:华东师范大学生命科学学院

摘要(Abstract)：

研究了大豆浓缩蛋白作为饵料蛋白源对中华绒螯蟹幼蟹消化酶活力的影响,初步探讨了中华绒螯蟹对饵料蛋白源的内在适应机理。根据中华绒螯蟹的营养需求,用大豆浓缩蛋白替代鱼粉作为蛋白源配制成6种近似等蛋白等能的饵料,其中大豆浓缩蛋白在饵料中的含量分别为0、11%、22%、33%、44%和56%(分别替代鱼蛋白质量的0、18.64%、37.93%、56.90%、77.19%和100%),对照饵料全部以鱼粉作为蛋白源。用上述6种饵料喂养中华绒螯蟹50 d后,测定蟹肝胰腺各种消化酶活力。结果表明:随着饵料中大豆浓缩蛋白含量的升高,胃蛋白酶和胰蛋白酶活力均呈降低之势。当大豆浓缩蛋白含量达到44%时,与全鱼粉对照组相比,胃蛋白酶和胰蛋白酶活力显著性降低(P<0.05);同时,淀粉酶稍呈升高之势,而纤维素酶则稍呈降低的趋势;A/T比例呈显著上升的趋势,当大豆浓缩蛋白含量达44%时,与对照组有显著性差异,说明河蟹对植物蛋白有很强的适应性。

关键词(KeyWords)： 大豆浓缩蛋白;中华绒螯蟹;消化酶;鱼粉

Abstract:

Keywords:

基金项目(Foundation): Supported by the national natural science foundation of China(30271012,30300265),the research fund for the doctoral programof higher education(20040269011),The programof Shanghai subject chief scientist(05xd4005)andthe Qinhuang-dao godensea foodindustry of Yihai group·

作者(Author): 李二超,陈立侨,蔡永久,顾顺樟,洪美玲,张璐,刘超

参考文献(References)：

• [1]Lovell R T.Use of soybean products in diets for aquaculturespecies[C]//Animal Nutrition Bulletin.American SoybeanAssociation,February,1984,1-6.
• [2]Regost C,Arzel J,Kaushik S J.Partial or total replacement offish meal by corn gluten meal in diet for turbot(Psetta maxi-ma)[J].Aquaculture,1999,180:99-117.
• [3]Elangovan Adrian,Shim K F.The influence of replacing fishmeal partially in the diet with soybean meal on growth andbody composition of juvenile tin foil barb(Barbodes altus)[J].Aquaculture,2000,189:133-144.
• [4]Kikuchi Kotaro.Use of defatted soybean meal as a substitutefor fish meal in diets of Japanese flounder(Paralichthys oliva-ceus)[J].Aquaculture,1999,179:3-11.
• [5]Storebakken T,Shearer K D,Roem A J.Availability of pro-tein,phosphorus and other elements in fish meal,soy-proteinconcentrate and phytase-treated soy-protein-concentrate-baseddiets to Atlantic salmon,Salmo salar[J].Aquaculture,1998,161:365-379.
• [6]Kaushik S J,Cravedi J P,Lalles J P,et al.Partial or total re-placement of fish meal by soy protein on growth,protein utili-zation,potential estrogenic or antigenic effects,cholesterol-emia and flesh quality in rainbow trout[J].Aquaculture,1995,133:257-274.
• [7]Escaffre A M,Zambonino Infante J L,Cahu C L,et al.Nutri-tional value of soy protein concentrate for larvae of commoncarpCyprinus carpiobased on growth performance and diges-tive enzyme activities[J].Aquaculture,1997,153:63-80.
• [8]Takii K,Shimeno S,Nakamura M,et al.Evaluation of soyprotein concentrate as a partial substitute for fish meal proteinin practical diet for yellowtail[C]//Takeda M,Watanabe T,ed.The current status of fish nutrition in aquaculture.TokyoUniv.of Fisheries,Tokyo,Japan,1990:281-288.
• [9]Stuart J S,Hung S S O.Growth of juvenile white sturgeonAcipenser transmontanusfed different proteins[J].Aquacul-ture,1989,76:303-316.
• [10]Forster L,Dominy W,Tacon.The use of concentrates andother soy products in shrimp feeds[C]//Cruz-Suarez L E,Ricque-Marie D,Tapia-Salazar M,et al,ed.Advaces en nu-tricion acuicola VI.Memorias del VI simposium internacionalde nutrcion acuicola.Cancun,Quintana Roo,Mexico,2002.
• [11]Paripatananont T,Boonyaratpalin M,Pengseng P,et al.Substitution of soy protein concentrate for fishmeal in diets oftiger shrimpPenaeus monodon[J].Aquaculture research,2001,32:369-374.
• [12]Biesot P M,Capuzzo J M.Changes in digestive enzyme activi-ties during early development of the American lobsterHoma-rus americanusMilne Edwards[J].J Exp Mar Biol Ecol,1990,136:107-122.
• [13]Johnston D J,Yellowlees D.Relationships between dietarypreferences and digestive enzyme complement of the slipperlobsterThenus orientalis(Decapoda:Scyllaridae)[J].JCrustacean Biol,1998,18:656-665.
• [14]Lee P G,Lawrence A L.Effects of diet and size on growth,feed digestibility and digestive enzyme activities of the marineshrimp,Penaeus setiferusLinnaeus[J].J world MariculSoc,1985,16:275-287.
• [15]Maugle P D,Deshimaru O,Catayama T,et al.Effect of shortnecked clam diets on shrimp growth and digestive enzyme ac-tivities[J].Bull Jpn Soc Sci Fish,1982,48:1759-1764.
• [16]Córdova-Murueta,J,García-Carre o F,Navarrete del T,etal.Digestive enzymes present in crustacean feces as a tool forbiochemical,physiological,and ecological studies[J].Journalof experimental marine biology and ecology,2003,297:43-56.
• [17]Le Moullac G,Van Wormhoudt A.Adaptation of digestiveenzymes to dietary protein,carbohydrate and fibre levels andinfluence of protein and carbohydrate quality inPenaeus van-nameilarvae(Crustacea,Decapoda)[J].Aquatic living re-sources,1994,7:203-210.
• [18]Lucien-Brun H,Van Wormhoudt A,Lachaux J A,et al.Effect de regime composés sur la croissance de homards juven-iles,Hommarus gammarus:La estimation biochimique de lacomposition optimale du régime alimentaire en proteínes[J].Aquaculture,1985,46:97-109.
• [19]Lee PG,Smith LL,Lawrence AL.Digestive proteases ofPenaeus vannameiBoone:relationship between enzyme activ-ities,size,and diet[J].Aquaculture,1984,42:225~239.
• [20]Lee P G,Lawrence A L.Effect of diet and size on growth,feed digestibility and digestive enzymes activities of the marineshrimp,Penaeus setiferusLinnaeus[J].Journal of the worldmariculture society,1985,16:275-287.
• [21]Kumlu M.Physiology of decapod crustacean larvae with spe-cial reference to diet[D].Bangor,UK:University of Wales,1995.256.
• [22]Jones D A,Kamarudin MS,Le Vay L.The potential for re-placement of live feeds in larval culture[J].J world AquacultSoc,1993,24(2):199-210.
• [23]Kanazawa A.Protein requirements of penaeid shrimp[C]//Advances in tropical aquaculture,1990.261-270.
• [24]Biesiot P M,Capuzzo J M.Change in digestive enzyme activi-ties during early development of the American lobster Hom-aus americanus[J].Mar Bio Ecol,1990,136(2):107-122.
• [25]Fange,R.&Grove,D.Digestion[M]//Hoar WS,RandallD J,Brett J R,ed.Fish Physiology(Vol.8).London:Aca-demic press,1979:161-260.
• [26]Ugolev A M,Yegorova V V,Kuz’mina V V,et al.Compar-ative molecular characterization of membrane digestion in fishand mammals[J].Comp Biochem Physiol B Biochem MolBiol,1983,76:627-635.
• [27]Kapoor B G,Smit H,Verighina I A.The alimentary canaland digestion in Teleosts[J].Ad Mar Biol,1975,13:109-239.