鱼干曲霉菌分离株鉴定及产毒特性Identification and Toxin-producing Characters of the Aspergillus in Dried Fish
王雅玲,宋颖慧,邱妹,陶森,叶林,邓义佳,房志家,孙力军,邓旗
摘要(Abstract)：
【目的】探明湛江市售鱼干中曲霉菌(Aspergillus spp.)分离株产AFB_1的特性。【方法】采用马铃薯-葡萄糖琼脂(PDA)对鱼干中的曲霉菌进行分离纯化,根据菌落形态、显微形态观察和ITS序列分析对分离株进行鉴定,采用液相色谱-串联质谱(LC-MS/MS)法分析菌株的AFB_1合成量。【结果】鱼干中曲霉菌的分离率高达47.66%,经形态学鉴定发现以黄曲霉(Aspergillus flavus)A03、烟曲霉(Aspergillus fumigatus)H32、杂色曲霉(Aspergillus versicolor)M23、欧式曲霉(Aspergillus europaeus)M11、棘孢曲霉(Aspergillus aculeatus)B21等为主,其中红笛鲷干(Lutjanus sanguinaus)中黄曲霉菌的含量高达1 200 CFU/g。黄曲霉A03在红笛鲷干培养基中常温(27～30℃)培养21 d,产AFB_1能力达到3.8 ng/mL。【结论】鱼干中存在产AFB_1的黄曲霉菌,警示鱼干中存在AFB1污染的风险。
关键词(KeyWords)： 鱼干;黄曲霉菌;AFB_1;ITS序列
基金项目(Foundation): 国家自然科学基金面上项目(31871898);; 广东省现代农业产业技术体系创新团队建设专项资金(No.2020KJ149;No.2020KJ151);; 湛江市科技计划项目(No.2020A03009;2020A01011);; 广东海洋大学科研启动经费资助项目(R19050)
作者(Author): 王雅玲,宋颖慧,邱妹,陶森,叶林,邓义佳,房志家,孙力军,邓旗
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