吕冉晖,钟燕华,游阳,袁润涵,关恩怡,段杉

• 1:华南农业大学食品学院广东省功能食品活性物重点实验室
• 2:北方伟业计量集团有限公司

摘要(Abstract)：

【目的】分析嗜盐四联球菌（Tetragenococcus halophilus）中耐盐和耐酸性能优劣菌株的核心基因家族差异，筛选可能与耐盐、耐酸性有关的代谢通路和基因功能。【方法】从高盐水产品和酱醪中筛选嗜盐四联球菌，采用16S rDNA测序方法进行鉴定，对分离出的17株嗜盐四联球菌进行生理生化鉴定和耐盐和耐酸能力的测试。分别将耐盐、耐酸性能差异明显的菌株进行分类，并进一步基于Illumina平台对17株菌株进行基因组测序，比较耐盐、耐酸性能差异明显的菌株的核心基因家族，再对耐盐、耐酸性能较好菌株的特有核心基因家族进行KEGG通路和GO功能的富集分析。【结果与结论】从高盐水产品和酱醪等不同来源分离的17株嗜盐四联球菌在生理生化鉴定中存在差异，但均对β-半乳糖苷和木糖表现为阴性，对果糖、山梨糖和D-核糖表现为阳性。耐受性结果显示，各菌株的耐受性具有显著差异。基因组测序结果表示，耐盐能力较好的菌株拥有294个特有核心基因家族，在KEGG通路中主要富集在磷酸戊糖途径、甲烷代谢、抗坏血酸和醛酸的新陈代谢以及磷酸转移酶系统（PTS），在GO功能中主要富集在定位、运输、建立定位、DNA重组和DNA代谢过程；耐酸能力较好的菌株拥有191个特有核心基因家族，在KEGG通路中主要富集在果糖和甘露糖代谢、淀粉和蔗糖代谢、类胡萝卜素的生物合成、氨基酸和核苷酸代谢以及PTS，在GO功能中主要富集在DNA重组和DNA代谢过程。上述通路和功能涵盖了部分与嗜盐四联球菌耐盐、耐酸特性相关的基因特征。进一步分析发现，两类特有核心基因家族富集在PTS中的基因完全一样，推测这些基因与嗜盐四联球菌的抗逆机制密切相关。

关键词(KeyWords)： 嗜盐四联球菌;耐盐能力;耐酸能力;分离;基因组测序;特有核心基因家族

Abstract:

Keywords:

基金项目(Foundation): 阳江市省科技创新战略专项（“大专项+任务清单”）项目（SDZX2023028）

作者(Author): 吕冉晖,钟燕华,游阳,袁润涵,关恩怡,段杉

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