杨桐林,黄洋,茹笑影,李锦,朱锟锋,杨佳慧,陈培培,朱春华

• 1:广东海洋大学水产学院
• 2:南方海洋科学与工程广东省实验室(湛江)
• 3:广东省名特优鱼类生殖调控与繁育工程技术研究中心
• 4:广东省海水养殖生物育种工程实验室

摘要(Abstract)：

【目的】探讨配合饲料替代冰鲜鱼条件下，高体(鱼师)（Seriola dumerili）幼鱼生长及肝脏组织代谢相关基因的表达，揭示配合饲料影响高体(鱼师)生长、代谢的分子机制，为其精准配合饲料开发提供参考。【方法】分别将以冰鲜鱼与配合饲料喂养60 d的高体(鱼师)幼鱼肝脏组织进行转录组测序分析，筛选差异表达基因（DEGs），富集分析GO和KEGG信号通路，RT-qPCR检测不同饵料喂养后高体(鱼师)肝脏中生长及代谢相关基因的表达。【结果】高体(鱼师)肝脏转录组测序获得高质量数据（clean reads）共247 272 212，各样品Q30均不低于94.11%；与冰鲜鱼组对比，配合饲料组共筛选到144个DEGs，其中81个DEGs表达上调，63个DEGs表达下调；KEGG通路富集分析发现，配合饲料组相对冰鲜鱼组显著富集在甾体生物合成、不饱和脂肪酸生物合成、萜类骨架生物合成、胆固醇代谢、脂肪代谢等脂质合成与代谢通路；转录组结果显示，配合饲料组与冰鲜鱼组肝脏中生长相关基因无显著差异，而代谢相关基因表达显著上调，RT-qPCR结果与转录组结果相一致。【结论】以配合饲料替代冰鲜鱼饲喂高体(鱼师)幼鱼，幼鱼生长差异不显著，但肝脏的脂质代谢能力显著提升。

关键词(KeyWords)： 高体(鱼师);饲料替代;转录组测序;脂质代谢

Abstract:

Keywords:

基金项目(Foundation): 海南省重大科技计划项目（ZDKJ2021011）

作者(Author): 杨桐林,黄洋,茹笑影,李锦,朱锟锋,杨佳慧,陈培培,朱春华

参考文献(References)：

• [1]廖志强.高体网箱养殖技术[J].中国水产, 2003(12):60-61.
• [2] PENG Y H, SHI H J, LIU Y Q, et al. RNA sequencing analysis reveals divergent adaptive response to hypo-and hyper-salinity in greater amberjack(Seriola dumerili)juveniles[J]. Animals, 2022, 12(3):327.
• [3]刘丽杰,彭凯.水产配合饲料替代冰鲜杂鱼的研究进展[J].饲料研究, 2021, 44(21):141-144.
• [4]李金秋,林建斌,朱庆国,等.人工配合饲料与小杂鱼饲养赤点石斑鱼效果的对比试验[J].台湾海峡, 2004, 23(2):167-173.
• [5]王广军.使用全价粉状饲料与冰鲜杂鱼喂海水鱼的效果对比试验[J].北京水产, 2000(3):26.
• [6]周兴,牛化欣.颗粒饲料和杂鱼对半滑舌鳎生长和饲料利用率的影响[J].饲料工业, 2020, 41(8):48-52.
• [7] REINKE H, ASHER G. Circadian clock control of liver metabolic functions[J]. Gastroenterology, 2016, 150(3):574-580.
• [8]石红娟,茹笑影,刘玉琪,等. 17β-雌二醇注射雌性金钱鱼下丘脑转录组分析[J].广东海洋大学学报, 2021, 41(2):76-85.
• [9] ARAKI K, AOKIC J Y, KAWASE J, et al. Whole genome sequencing of greater amberjack(Seriola dumerili)for SNP identification on aligned scaffolds and genome structural variation analysis using parallel resequencing[J]. International Journal of Genomics, 2018, 2018:1-12.
• [10] RILEY L G, WALKER A P, DOROUGH C P, et al. Glucose regulates ghrelin, neuropeptide Y, and the GH/IGF-I axis in the tilapia, Oreochromis mossambicus[J]. Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology, 2009, 154(4):541-546.
• [11] FOX B K, BREVES J P, DAVIS L K, et al. Tissue-specific regulation of the growth hormone/insulin-like growth factor axis during fasting and re-feeding:importance of muscle expression of IGF-I and IGF-II mRNA in the tilapia[J].General and Comparative Endocrinology, 2010, 166(3):573-580.
• [12] PEDROSO F L, DE JESUS-AYSON E G T, CORTADO H H, et al. Changes in mRNA expression of grouper(Epinephelus coioides)growth hormone and insulin-like growth factor I in response to nutritional status[J]. General and Comparative Endocrinology, 2006, 145(3):237-246.
• [13]陈乃松,周洁,靳利娜,等.禁食对大口黑鲈生长和肝脏IGF-ⅠmRNA表达丰度的影响[J].中国水产科学, 2010,17(4):713-720.
• [14]沈伟良,钱宝英,薛良义.饥饿和复投喂对大黄鱼(Larimichthys crocea)IGF-Ⅰ、mTOR、MyoD和MHC基因表达的影响[J].海洋与湖沼, 2019, 50(4):894-902.
• [15]曾延清,王立改,徐冬冬,等.投喂配合饲料和冰鲜杂鱼对黄姑鱼生长性能、饲料利用、生长相关基因表达和养殖水质的影响[J].浙江海洋大学学报(自然科学版),2022, 41(4):299-307; 314.
• [16] DUAN C, DUGUAY S J, PLISETSKAYA E M. Insulin-like growth factor I(IGF-I)mRNA expression in coho salmon,Oncorhynchus kisutch:tissue distribution and effects of growth hormone/prolactin family proteins[J]. Fish Physiology and Biochemistry, 1993, 11(1/2/3/4/5/6):371-379.
• [17] DUAN C, HIRANO T. Effects of insulin-like growth factor-I and insulin on the in-vitro uptake of sulphate by eel branchial cartilage:evidence for the presence of independent hepatic and pancreatic sulphation factors[J]. The Journal of Endocrinology, 1992, 133(2):211-219.
• [18] CHEN J Y, CHEN J, CHANG C Y, et al. Expression of recombinant tilapia insulin-like growth factor-I and stimulation of juvenile tilapia growth by injection of recombinant IGFs polypeptides[J]. Aquaculture, 2000, 181:347-360.
• [19] BUNLIPATANON P, SONGSEECHAN N, KONGKEO H, et al. Comparative efficacy of trash fish versus compounded commercial feeds in cage aquaculture of Asian seabass(Lates calcarifer)(Bloch)and tiger grouper(Epinephelus fuscoguttatus)(Forssk?l)[J]. Aquaculture Research, 2014, 45(3):373-388.
• [20]阙有清,杨志刚,纪连元,等.配合饲料替代杂鱼对中华绒螯蟹生长发育、体成分及脂肪酸组成的影响[J].水产学报, 2012, 36(10):1612-1623.
• [21]李婷婷,褚志鹏,李创举,等.饲料中不同脂肪源对杂交鲟幼鱼生长性能、体成分、养分表观消化率、肝脏脂肪代谢酶活性和血清生化指标的影响[J].动物营养学报,2021, 33(6):3447-3460.
• [22] QIU H J, JIN M, LI Y, et al. Dietary lipid sources influence fatty acid composition in tissue of large yellow croaker(Larmichthys crocea)by regulating triacylglycerol synthesis and catabolism at the transcriptional level[J]. PloS One,2017, 12(1):e0169985.
• [23]黄岩,李建,王学习,等.饲料中不同蛋白质和淀粉水平对斜带石斑鱼生长性能和肝脏相关代谢酶活性的影响[J].水产学报, 2017, 41(5):746-756.
• [24]朱筛成,龙晓文,向朝林,等.复合蛋白源替代鱼粉对中华绒螯蟹幼蟹生长性能、生理代谢和生化组成的影响[J].南方水产科学, 2019, 15(2):83-92.
• [25] HASBARGEN KATHRIN B, SHEN W J, ZHANG Y Q,et al. Slc43a3 is a regulator of free fatty acid flux[J]. Journal of Lipid Research, 2020, 61(5):734-745.
• [26]李佩韦,昝林森.秦川牛PLIN2基因参与脂滴形成调节机制的研究[J].西北农林科技大学学报(自然科学版),2023, 51(4):1-7.
• [27] LONG T, LIU Y, LI X C. Molecular structures of human ACAT2 disclose mechanism for selective inhibition[J].Structure, 2021, 29(12):1410-1418.e4.
• [28] MA Z, HUANG Z, ZHANG C, et al. Hepatic Acat2 overexpression promotes systemic cholesterol metabolism and adipose lipid metabolism in mice[J]. Diabetologia, 2023,66(2):390-405.