杜冉,陈忠琴,谭明堂,高加龙,林海生,郑惠娜,曹文红

• 1:广东海洋大学食品科技学院/国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/广东省海洋食品工程技术研究中心/水产品深加工广东普通高等学校重点实验室

摘要(Abstract)：

【目的】探究香港牡蛎（Crassostrea hongkongensis）锌结合肽（ZnPE）在热环境、酸碱环境中的稳定性及跨Caco-2细胞模型的吸收转运机制，为锌补剂开发提供理论依据。【方法】以牡蛎锌螯合肽（Zn-PE）和ZnSO_4为对照，以总锌、锌离子的溶解率为评价指标，在40、50、60、70和80℃以及pH值为2、4、6、8、10条件下，研究ZnPE的热稳定性和酸碱稳定性；并建立Caco-2细胞模型，研究ZnPE在跨Caco-2细胞模型的吸收率、生物利用率和转运途径；通过液质联用法研究ZnPE跨Caco-2细胞模型后的序列变化。【结果】不同温度中ZnPE与Zn-PE的总锌、锌离子溶解率无显著差异（P>0.05），两者均可保持较高的稳定性。在酸性环境（pH=2）中，ZnPE和Zn-PE中总锌溶解率均在90%以上，而锌离子有一定程度的释放，溶解率分别为22.30%、33.45%，两者稳定程度降低，但ZnPE稳定性显著高于Zn-PE(P<0.05）。在碱性环境（pH=8）中，ZnPE和Zn-PE均保持稳定。ZnPE可完整、稳定的通过Caco-2细胞模型，且其锌的吸收率为74.37%、生物利用率为76.40%，显著高于Zn-PE、ZnSO_4(P<0.05）。ZnPE中的锌主要以肽的形式通过细胞旁路和转胞吞作用的方式跨Caco-2细胞模型转运。转运后鉴定出8种锌结合肽，其中的5种和转运前一致，表明ZnPE在吸收转运中可保持较高的稳定性。【结论】ZnPE可在热环境、酸碱环境中及跨Caco-2细胞模型转运时保持较高的稳定性。

关键词(KeyWords)： 牡蛎;锌结合肽;稳定性;Caco-2细胞;吸收转运

Abstract:

Keywords:

基金项目(Foundation): 湛江市农业技术攻关项目（2022A05038）

作者(Author): 杜冉,陈忠琴,谭明堂,高加龙,林海生,郑惠娜,曹文红

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