梁海鹰,陈洁,张美珍

• 1:广东海洋大学水产学院
• 2:广东省水产动物病害防控与健康养殖重点实验室

摘要(Abstract)：

【目的】研究蛋白质琥珀酰化修饰在马氏珠母贝（Pinctada fucata martensii）植核后免疫应答中的作用，为阐明珍珠贝植核反应的分子调控机制提供理论依据。【方法】通过对马氏珠母贝注射琥珀酸钠模拟提高琥珀酰化修饰水平，检测马氏珠母贝植核后鳃组织总蛋白琥珀酰化程度、免疫相关基因表达、抗氧化相关酶酶活以及珍珠贝的存活率和留核率。【结果与结论】注射琥珀酸钠后，鳃组织琥珀酰化修饰水平在12 h和72 h显著上升；使用实时荧光定量PCR检测胁迫应激相关基因的时序表达，胁迫应激相关因子SOD和Caspase2在6 h表达量显著上调，NF-κB和IRAK1在48 h表达量显著上调，TRAF3和IκK的表达量在96 h显著上调（P <0.05），表明琥珀酰化修饰水平提高后受体贝机体细胞免疫增强；抗氧化相关酶、过氧化物酶和谷胱甘肽活性都在96 h出现显著上调（P <0.05），表明受体贝体液免疫增强。与对照组[磷酸盐缓冲溶液（PBS）+植核]和植核组相比，实验组（琥珀酸钠+植核）中珍珠贝的存活率在7 d和45 d存在显著差异，在60 d留核率显著上升（P <0.05）。结果说明，琥珀酰化修饰参与了马氏珠母贝的植核免疫反应，注射琥珀酸钠可以提高受体贝的免疫活力，并提高其在植核后的存活率和留核率。

关键词(KeyWords)： 马氏珠母贝;植核免疫;琥珀酰化修饰;琥珀酸钠

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金项目(2023A1515012924,2021A1515010962);; 广东省科技专项资金(2021A05250);; 广东省海港建设与渔业产业发展专项(A201608B15);; 南海经济动物种质创新与利用创新团队项目(2021KCXTD026)

作者(Author): 梁海鹰,陈洁,张美珍

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