欧泽奎,刘东超,麦铭雄,何宇轩,杨鹏

• 1:广东海洋大学水产学院

摘要(Abstract)：

在黑暗条件下对全缘马尾藻(Sargassum integerrimum)幼孢子分别进行0.5、1、2、4 h干出处理,研究干出处理对幼孢子体生长、幼孢子体体内叶绿素a、叶绿素c、类胡萝卜素、脯氨酸以及可溶性糖含量的影响。结果表明:相比于未进行干出处理的对照组,0.5 h、1 h组的短暂干出可促进幼孢子体生长(P<0.05),幼孢子体体内两种叶绿素、类胡萝卜素、脯氨酸以及可溶性糖含量差异均无统计学意义(P>0.05);2 h、4 h组的长时间干出可抑制幼孢子体生长(P<0.05),幼孢子体体内两种叶绿素含量均降低(P<0.05),类胡萝卜素、脯氨酸以及可溶性糖的含量升高(P<0.05)并保持较高水平。在人工育苗过程中每天在黑暗条件下对幼孢子体进行短时间(0.5~1 h)干出处理,可促进幼孢子体生长,并适当提高其抗逆性以及对干旱的适应性。

关键词(KeyWords)： 全缘马尾藻;幼孢子体;干出;生长;生化成分

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目(2006B60501016);广东省科技计划项目(2009B080701048)

作者(Author): 欧泽奎,刘东超,麦铭雄,何宇轩,杨鹏

参考文献(References)：

• [1]曾呈奎,陆保仁.中国海藻志·褐藻门·墨角藻目[M].北京:科学出版社,2000:170-171.
• [2]卢虹玉,杨小青,谢恩义,等.全缘马尾藻的主要营养成分分析与评价[J].食品研究与开发,2013,34(7):120-122.
• [3]JIN W H,ZHANG W J,WANG J,et al.A study of neuroprotective and antioxidant activities of heteropolysaccharides from six Sargassum species[J].Int J Bio Macromol,2014,67:336-342.
• [4]卢虹玉,刘义,吉宏武,等.全缘马尾藻褐藻多酚的抗氧化和抗肿瘤细胞增殖作用研究[J].现代食品科技,2013,29(4):702-705.
• [5]肖为,陶叶杏,谷毅鹏,等.全缘马尾藻提取物对酵母膏诱导小鼠高尿酸血症的拮抗效应[J].食品工业科技,2015,36(17):339-342.
• [6]黄苑媚,刘志刚,谢恩义,等.水流速率对全缘马尾藻幼孢子体生长和生理活性的影响[J].广东海洋大学学报,2014,34(6):45-50.
• [7]孙宗红,麦惠欣,刘志刚,等.温度对全缘马尾藻幼孢子体生长和生理组分的影响[J].广东海洋大学学报,2015,35(1):51-56.
• [8]吴倩倩,佟少明,侯和胜.干出胁迫对孔石莼生长及生理影响[J].海洋渔业,2011,33(4):472-475.
• [9]郭赣林,董双林.干出对潮间带不同垂直位置海藻的生长及光合作用速率的影响[J].海洋湖沼通报,2008(4):78-84.
• [10]邹定辉,高坤山.低潮干出状态下石莼的光合作用特性(简报)[J].植物生理学通讯,2001,37(6):503-506.
• [11]KUMAR M,GUPTA V,TRIVEDI N,et al.Desiccation induced oxidative stress and its biochemical responses in intertidal red alga Gracilaria corticata(Gracilariales,Rhodophyta)[J].Environ Exp Bot,2011,72(2):194-201.
• [12]VAN HEES D H,VAN ALSTYNE K L.Effects of emersion,temperature,dopamine,and hypoxia on the accumulation of extracellular oxidants surrounding the bloom-forming seaweeds Ulva lactuca and Ulvaria obscura[J].J Exp Mar Bio Ecol,2013,448:207-213.
• [13]郭赣林,阎斌伦,徐静,等.连续性干出对孔石莼生长和生化组成的影响[J].生态学杂志,2011,30(12):2767-2769.
• [14]YAMOCHI S.Effects of desiccation and salinity on the outbreak of a green tide of Ulva pertusa in a created salt marsh along the coast of Osaka Bay,Japan[J].Estuar Coast Shelf S,2013,116:21-28.
• [15]陈昌生,翁琳,汪磊,等.干露和冷藏对坛紫菜及杂藻存活与生长的影响[J].海洋学报(中文版),2007,29(2):131-136.
• [16]张芬来.干露影响坛紫菜叶状体生理活性和生长的试验[J].浙江水产学院学报,1993,12(3):204-211.
• [17]杨帆,李世国,佟少明,等.黑暗条件下干露对孔石莼生理生化指标的影响[J].延边大学学报(自然科学版),2012,38(1):54-58.
• [18]CONTRERAS-PORCIA L,CALLEJAS S,THOMAS D,et al.Seaweeds early development:detrimental effects of desiccation and attenuation by algal extracts[J].Planta,2012,235(2):337-348.
• [19]贾柽,杨彬,谢恩义.硇洲马尾藻人工育苗常见敌害生物及防治初探[J].水产养殖,2012,33(7):35-39.
• [20]ALTAMIRANO M,FLORES-MOYA A,FIGUEROA F L.Effects of UV radiation and temperature on growth of germlings of three species of Fucus(Phaeophyceae)[J].Aquat Bot,2003,75(1):9-20.
• [21]CHU S H,ZHANG Q S,LIU S K,et al.Tolerance of Sargassum thunbergii germlings to thermal,osmotic and desiccation stress[J].Aquat Bot,2012,96(1):1-6.
• [22]JEFFREY S W,Humphrey G F.New spectrophotometric equations for deteming Chlorophylls a,b,c1 and c2 in higher plants algae and natural phytoplankton[J].Biochem Physiol Pflanzen,1975,167:191-199.
• [23]朱广廉,邓兴旺,左卫能.植物体内游离脯氨酸的测定[J].植物生理学通讯,1983(1):35-37.
• [24]丁雪梅,张晓君,赵云,等.蒽酮比色法测定可溶性糖含量的试验方法改进[J].黑龙江畜牧兽医,2014(12):230-233.
• [25]FLORES-MOLINA M R,THOMAS D,LOVAZZANO C,et al.Desiccation stress in intertidal seaweeds:Effects on morphology,antioxidant responses and photosynthe-tic performance[J].Aquat Bot,2014,113:90-99.
• [26]SCHAGERL M,M?STL M.Drought stress,rain and recovery of the intertidal seaweed Fucus spiralis[J].Mar Biol,2011,158(11):2471-2479.
• [27]LAMOTE M,JOHNSON L E,LEMOINE Y.Photosynthetic responses of an intertidal alga to emersion:The interplay of intertidal height and meteorological conditions[J].J Exp Mar Bio Ecol,2012,428:16-23.
• [28]邹定辉,阮祚禧,陈伟洲.干出状态下羊栖菜的光合作用特性[J].海洋通报,2004,23(5):33-39.
• [29]邹定辉,高坤山.低潮干出状态下潮间带海藻的某些光合生理特性[J].植物生理学通讯,2003,39(5):525-530.
• [30]SAMPATH-WILEY P,NEEFUS C D,JAHNKE L S.Seasonal effects of sun exposure and emersion on intertidal seaweed physiology:Fluctuations in antioxidant contents,photosynthetic pigments and photosynthetic efficiency in the red alga Porphyra umbilicalis Kützing(Rhodophyta,Bangiales)[J].J Exp Mar Bio Ecol,2008,361(2):83-91.
• [31]FLORES-MOLINA M R,THOMAS D,LOVAZZANO C,et al.Desiccation stress in intertidal seaweeds:Effects on morphology,antioxidant responses and photosynthetic performance[J].Aquat Bot,2014,113:90-99.
• [32]何培民,秦松,严小军,等.海藻生物技术及其应用[M].北京:化学工业出版社,2007:221.
• [33]TAKASHIMA M,SHICHIRI M,HAGIHARA Y,et al.Capacity of fucoxanthin for scavenging peroxyl radicals and inhibition of lipid peroxidation in model systems[J].Free Radic Res,2012,46(11):1406-1412.
• [34]FUNG A,HAMID N,LU J.Fucoxanthin content and antioxidant properties of Undaria pinnatifida[J].Food Chem,2013,136(2):1055-1062.
• [35]赵雅静,翁伯琦,王义祥,等.植物对干旱胁迫的生理生态响应及其研究进展[J].福建稻麦科技,2009,27(2):45-50.
• [36]LIU H,ZHANG Y,YIN H,et al.Alginate oligosaccharides enhanced Triticum aestivum L.tolerance to drought stress[J].Plant Physiol Bioch,2013,62:33-40.
• [37]钱飞箭.坛紫菜受高温和干出胁迫的机理及脂代谢关键基因的研究[D].宁波:宁波大学,2014.
• [38]赵素达,董树刚,吴以平,等.盐胁迫对孔石莼的生理生化影响[J].海洋科学,2000,24(7):52-55.