口蹄疫病毒VP1植物表达载体的构建Construction of VP1 Plant Expression Vector of Foot-and-Mouth Disease Virus
刘铀,毕英佐,马静云,曹永长
摘要(Abstract)：
P1 T重组质粒上含有口蹄疫病毒 (FMDV)GD10分离株的p1cDNA片段 ,以此为模板 ,用PCR方法扩增其中的VP1基因 ,获得大小约 6 40bp的片段。该片段用BglⅡ和BstEⅡ酶切消化后克隆至表达载体 pCAMBIA130 5 .2 ,转化EcoliTOP10感受态细胞。重组质粒经PCR、酶切及序列分析 ,证实VP1基因处于CaMV35S启动子控制 ,且读码框正确
关键词(KeyWords)： 口蹄疫病毒;VP1基因;植物表达载体
基金项目(Foundation):
作者(Author): 刘铀,毕英佐,马静云,曹永长
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