杨青峰,李雁群,许英桃,张茜,杨慧敏,钟敏,胡雪琼,PEI Yi

• 1:广东海洋大学食品科技学院//海洋药物研究所//广东省水产品加工与安全重点实验室
• 2:College of Food Science and Technology
• 3: Research Institute for Marine Drugs and Nutrition
• 4:Guangdong Ocean University//Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety

摘要(Abstract)：

用改良SE培养基培养富油新绿藻(Neochloris oleoabundans),用缺氮改良SE培养基培养处于稳定期后期的藻细胞3 d,分别收集处于生长期、稳定期、衰亡期和缺氮胁迫状态的藻细胞,用Folch法提取藻细胞油脂,以质量法计算油脂产率,用气相色谱法测定脂肪酸组成。结果表明,富油新绿藻随着培养期延长油脂含量提高,在缺氮胁迫状态下藻细胞油脂质量分数达28.99%;随着细胞油脂含量的增加,油脂脂肪酸组成也发生变化,从生长期到氮缺乏胁迫状态油酸质量分数由24%增至44%,而亚油酸质量分数由26.84%降至19.85%,亚麻酸质量分数由12.74%降至4.75%。富油新绿藻在分批培养后期和氮缺乏而碳源充足的条件下可积累油脂,而在油脂积累阶段油脂的脂肪酸组成亦不同。

关键词(KeyWords)： 富油新绿藻;油脂;脂肪酸;培养期

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金(2015A030313616);; 湛江市科技计划项目(2015A03026)

作者(Author): 杨青峰,李雁群,许英桃,张茜,杨慧敏,钟敏,胡雪琼,PEI Yi

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