王妹,邓思平,陈华谱,朱春华,吴天利,李广丽,江东能

• 1:广东海洋大学水产学院//南海水产经济动物增养殖广东省普通高校重点实验室//广东省名特优鱼类生殖调控与繁育工程技术研究中心工程技术研究中心//湛江市海洋生态与养殖环境重点实验室

摘要(Abstract)：

【目的】Amh(anti-Müllerian hormone)与其特异的Ⅱ型受体Amhr2共同参与脊椎动物性腺发育过程,本研究旨在了解金钱鱼Amhr2在性腺发育中的作用。【方法】克隆金钱鱼(Scatophagus argus)Amhr2,采用逆转录PCR和实时荧光定量PCR分别检测Amhr2的组织分布及其卵巢发育过程中的表达。【结果】金钱鱼Amhr2的开放阅读框(ORF)全长为1 533 bp,编码510个氨基酸。氨基酸序列分析表明,金钱鱼Amhr2 N端有信号肽、跨膜螺旋区和配体结合结构域。金钱鱼Amhr2与欧洲海鲈(Dicentrarchus labrax)的相似性最高,为73.1%,与人类的相似性最低,为23.2%。金钱鱼与欧洲海鲈和大黄鱼(Larimichthys crocea)亲缘关系最近,与进化地位一致,说明金钱鱼Amhr2与其他物种具有一定保守性。金钱鱼Amhr2主要在性腺表达,且精巢表达高于卵巢。Amhr2在Ⅱ时相卵巢中的表达显著高于ⅡI和IV时相卵巢。【结论】金钱鱼Amhr2序列与鲈形目同源性高,在雌雄性腺均发挥作用。

关键词(KeyWords)： 金钱鱼;Amhr2;基因克隆;基因表达;卵巢发育

Abstract:

Keywords:

基金项目(Foundation): 湛江市财政资金科技竞争性分配项目(2016A03017);; 广东省自然科学基金(2016A030313743);; 国家青年基金(31702326)

作者(Author): 王妹,邓思平,陈华谱,朱春华,吴天利,李广丽,江东能

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