军曹鱼全人工繁殖群体遗传特征的SSR分析A Genetic Analysis of Cultured Populations of Cobia (Rachycentron canadum) with Microsatellite Markers
王中铎,陈铁妹,郭昱嵩,罗杰,刘丽,刘楚吾
摘要(Abstract)：
利用8个微卫星DNA位点分析南海海域5个军曹鱼全人工繁育群体(HN1、HN2、ZJ、FJ和LS)子代的遗传多样性特征和群体间遗传分化。结果显示,军曹鱼养殖群体与天然群体的遗传结构特征基本一致:1)平均有效等位基因数为3.910±0.440,观测杂合度为0.595±0.049,分子方差分析(AMOVA)表明,个体内分化占主导(46%),军曹鱼养殖群体整体遗传多样性较高;2)群体间基因流明显(N_m=2.5959,F_(st)=0.0878),整体分化程度较低。各养殖群体表现出不同于天然大群体的特征:1)绝大部分位点均明显偏离哈温平衡,杂合子缺失或过剩现象普遍存在;2)聚类和群体分配分析等表明HN2与另四个养殖群体(HN1、ZJ、FJ和LS)分化明显。
关键词(KeyWords)： 军曹鱼;养殖群体;微卫星DNA;遗传多样性
基金项目(Foundation): “十一五”国家科技支撑计划项目(2007BAD29B03)
作者(Author): 王中铎,陈铁妹,郭昱嵩,罗杰,刘丽,刘楚吾
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